Biotechnology Techniques

, Volume 12, Issue 1, pp 25–30

Long-term cultivation of immortalised mouse hepatocytes in a high cell density, fixed-bed reactor

  • D. Fassnacht
  • S. Rossing
  • J. Stange
  • R. Portner
Article

DOI: 10.1023/A:1008895207570

Cite this article as:
Fassnacht, D., Rossing, S., Stange, J. et al. Biotechnology Techniques (1998) 12: 25. doi:10.1023/A:1008895207570

Abstract

An immortalised mouse hepatocyte line was grown in culture flasks and in a small-scale fixed-bed system to determine growth characteristics and a suitable carrier type. The cells were then cultivated in a 40 ml fixed-bed reactor over 75 days at a perfusion rate of 6.25 ml medium per ml fixed-bed and day. A cell density of approx. 8.5.107 cells per ml carrier was reached at the end of the experiment proving that a stable cultivation was possible over a long period of time with constant consumption and production rates.

Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • D. Fassnacht
  • S. Rossing
  • J. Stange
  • R. Portner

There are no affiliations available

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