Long-term cultivation of immortalised mouse hepatocytes in a high cell density, fixed-bed reactor
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- Fassnacht, D., Rossing, S., Stange, J. et al. Biotechnology Techniques (1998) 12: 25. doi:10.1023/A:1008895207570
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An immortalised mouse hepatocyte line was grown in culture flasks and in a small-scale fixed-bed system to determine growth characteristics and a suitable carrier type. The cells were then cultivated in a 40 ml fixed-bed reactor over 75 days at a perfusion rate of 6.25 ml medium per ml fixed-bed and day. A cell density of approx. 8.5.107 cells per ml carrier was reached at the end of the experiment proving that a stable cultivation was possible over a long period of time with constant consumption and production rates.