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Letters in Peptide Science

, Volume 4, Issue 4–6, pp 391–395 | Cite as

Micro-environment effect on the structure of synthetic substrates of thermolysin

  • Sylvie Baltora-Rosset
  • Danielle Marty
  • François-Yves Dupradeau
  • Emmanuel Pauthe
  • Véronique Larreta-Garde
  • Gilles Gacel
  • Jean-Pierre Monti
Article

Abstract

The conformations of thermolysin synthetic substrates inH2O/D2O (9/1) and glycerol-d5 (5 M) are investigated using two-dimensional nuclearmagnetic resonance spectroscopy and molecular modeling. The structuresobtained from molecular modeling and NMR studies are compared. Comparisonsof these structures with bound inhibitor in the active site of thermolysinare also discussed.

Molecular modeling NMR Peptides 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Sylvie Baltora-Rosset
    • 1
  • Danielle Marty
    • 1
  • François-Yves Dupradeau
    • 1
  • Emmanuel Pauthe
    • 2
  • Véronique Larreta-Garde
    • 2
  • Gilles Gacel
    • 1
  • Jean-Pierre Monti
    • 1
  1. 1.Groupe de Recherche des Biomolécules: Micro-environnement et MétabolismeFaculté de PharmacieAmiens Cedex 1France
  2. 2.Département de BiologieUniversité Cergy-PontoiseCergy-Pontoise CedexFrance

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