Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol
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Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-d-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-d-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.
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- Esterbauer, H., Steiner, W., Labudova, I., Hermann, A. & Hayn, M. 1991 Production of Trichoderma cellulase at laboratory and pilot scale. Biosource Technology 36, 51–65.Google Scholar
- Eveleigh, D.E. 1987 Cellulase-a perspective. Philosophical Transactions of the Royal Society, London A321, 435–447.Google Scholar
- Ghose, T.K. 1987 Measurement of cellulase activities. Pure and Applied Chemistry 59, 257–268.Google Scholar
- Nieves, R.A., Himmel, M.E., Todd, R.J. & Ellis, R.P. 1990 Crossreactive and specific monoclonal antibodies against cellobiohydrolase I, cellobiohydrolase II, endoglucanase I, and endoglucanase II of Trichoderma reesei. Applied and Environmental Microbiology 56, 1103–1108.Google Scholar
- Wood, T.M. 1971 The Cellulase of Fusarium solani: purification and specificity of the ß-(1,4)-glucanase and the ß-D-glucosidase components. Biochemical Journal 121, 353–362.Google Scholar
- Wright, J.D., Wyman, C.E. & Grohmann, K. 1988 Simultaneous saccharification and fermentation of lignocellulose: process evaluation. Applied Biochemistry and Biotechnology 17, 75–90.Google Scholar