Transgenic Research

, Volume 7, Issue 2, pp 105–112

Maternally expressed PGK-Cre transgene as a tool for early and uniform activation of the Cre site-specific recombinase

  • Yvan Lallemand
  • Victor Luria
  • Rebecca Haffner-Krausz
  • Peter Lonai
Article

Abstract

A transgenic mouse strain with early and uniform expres sion of the Cre site-specific recombinase is described. In this strain, PGK-Crem, Cre is driven by the early acting PGK-1 promoter, but, probably due to cis effects at the integration site, the recombinase is under dominant maternal control. When Cre is transmitted by PGK-Crem females mated to males that carry a reporter transgene flanked by loxP sites, even offspring that do not inherit PGK-Cre delete the target gene. It follows that in the PGK-Crem female Cre activity commences in the diploid phase of oogenesis. In PGK-Crem crosses complete recombination was observed in all organs, including testis and ovary. We prepared a mouse stock that is homozygous for PGK-Crem and at the albino (c) locus. This strain will be useful for the early and uniform induction of ectopic and dominant negative mutations, for the in vivo removal of selective elements from targeted mutations and in connection with the manipulation of targeted loci in 'knock in' and related technologies

Cre recombinase maternal expression gametogenesis unifrorm Cre activity early development 

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Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Yvan Lallemand
    • 1
  • Victor Luria
    • 1
  • Rebecca Haffner-Krausz
    • 1
  • Peter Lonai
    • 1
  1. 1.Department of Molecular GeneticsThe Weizmann Institute of ScienceRehovotIsrael

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