Abstract
A citric acid-producing strain of Aspergillus niger, grown on olive cake-based medium, was able to solubilize rock phosphate. Solubilization of insoluble phosphate increased during the solid-state fermentation process, reaching a maximum of 164μg/ml. Various combinations of olive cake and rock phosphate, previously treated or untreated by the fungus, were introduced into a calcareous, phosphorus (P)-deficient soil to improve the growth of Trifolium repens in a greenhouse experiment. Synergistic action of both the filamentous and arbuscular fungi caused considerable improvement of growth and plant P uptake. Greater growth and P uptake of mycorrhizal and non-mycorrhizal plants were achieved when microbe-treated olive cake and rock phosphate were applied to soil compared with all other treatments.
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Vassileva, M., Vassilev, N. & Azcon, R. Rock phosphate solubilization by Aspergillus niger on olive cake-based medium and its further application in a soil–plant system. World Journal of Microbiology and Biotechnology 14, 281–284 (1997). https://doi.org/10.1023/A:1008858802855
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DOI: https://doi.org/10.1023/A:1008858802855