Effect of fungicides, insecticides and allosamidin on a thermostable chitinase from Bacillus sp. BG-11

  • Bharat Bhushan
  • G.S. Hoondal
Article

Abstract

A purified, thermostable chitinase from Bacillus sp. BG-11 retained 90% of its activity in the presence of fungicides such as Aromex, Captafol, Captan, Chlorothalonil, Dinocap, Metalaxyl, sulphur, Triadimefon, and insecticides such as Acephate, Chloropyriphos, Cypermethrin, Diclovorus, Dimethoate, Methomyl, Malathion, Methylparathion and Monocrotophos at a concentration of 100 μg active ingredient per ml of enzyme solution. Allosamidin inhibited the chitinase with IC50 value of 48 μM.

Allosamidin Bacillus chitinase fungicide insecticide 

References

  1. Bhushan, B. & Hoondal, G.S. 1998 Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. BG-11. Biotechnology Letters. (Accepted, In Press).Google Scholar
  2. Dickinson, K., Keer, V., Hitchcock, C.A. & Adams, D.J. 1989 Chitinase activity from Canadian albicans and its inhibition by Allosamidin. Journal of General Microbiology 135, 1417–1421.PubMedGoogle Scholar
  3. Escott, G.M. & Adams, D.J. 1995 Chitinase activity in human serum and leucocytes. Infection and Immunity 63, 4770–4773.PubMedGoogle Scholar
  4. Gupta, R., Saxena, R.K., Chaturvedi, P. & Virdi, J.S. 1995 Chitinase production by Streptomyces virifificans: Its potential in fungal cell wall lysis. Journal of Applied Bacteriology 87, 378–383.Google Scholar
  5. Koga, D., Isogai, A., Sakuda, S., Matsumoto, S., Susuki, A., Kimura, S. & Ide, A. 1987 Specific inhibition of Bombyx mori chitinase by allosamidin. Agricultural and Biological Chemistry 51, 471–476.Google Scholar
  6. Mahadevan, B. & Crawford, D.L. 1997 Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC 108. Enzyme and Microbial Technology 20, 489–493.Google Scholar
  7. Pedraza-Reyes, M. & Lopez-Romero, E. 1991 Chitinase activity in germinating cells of Mucor rouxi. Antonie Van Leeuwenhoek 59, 183–189.PubMedGoogle Scholar
  8. Pinto, A.D.S., Barreto, C.C., Schrank, A., Ulhoa, C.J. & Vainstein, M.H. 1997 Purification and characterization of an extracellular chitinase from the entomopathogen Metarhizium anisopliae. Canadian Journal of Microbiology 43, 322–327.Google Scholar
  9. Reissig, J.L., Strominger, J.L. & Leloir, L.F. 1955 A modified colorimetric method for the estimation of N-acetylamino sugars. Journal of Biological Chemistry 217, 959–966.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Bharat Bhushan
    • 1
  • G.S. Hoondal
    • 1
  1. 1.Department of MicrobiologyPunjab UniversityChandigarh –India

Personalised recommendations