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Letters in Peptide Science

, Volume 4, Issue 4–6, pp 489–495 | Cite as

Structural modifications of oxidized insulin B-chain induced by glycerol addition

  • François-Yves Dupradeau
  • Guillaume Le Flem
  • Jean-Michel Wieruszeski
  • Manuel Dauchez
  • Alain Alix
  • Véronique Larreta-Garde
  • Jean-Pierre Monti
Article
  • 31 Downloads

Abstract

H-NMR studies of the bovine insulin S-sulfonatedB-chain are reported in H2O/D2O (9/1) and inglycerol-d5 (5 M) using two-dimensional NMRspectroscopy. The first results show that the oxidizedinsulin B-chain secondary structure differs from thatof native insulin by a loss of the α-helixbetween the two disulfide bridges and that theglycerol favours the structuring of the peptide.

CD Endopeptidase Micro-environment NMR Subtilisin BPN′ 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • François-Yves Dupradeau
    • 1
  • Guillaume Le Flem
    • 1
  • Jean-Michel Wieruszeski
    • 2
  • Manuel Dauchez
    • 3
  • Alain Alix
    • 3
  • Véronique Larreta-Garde
    • 4
  • Jean-Pierre Monti
    • 1
  1. 1.Groupe de recherche des biomolécules: Micro-environnement et MétabolismeFaculté de PharmacieAmiens Cedex 1Franc
  2. 2.Laboratoire de RMN, URA CNRS 1309Institut PasteurFrance
  3. 3.Laboratoire de Spectroscopies et Structures BiomoléculairesReimsFrance
  4. 4.Laboratoire de Technologie EnzymatiqueCompiègneFrance

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