Biotechnology Techniques

, Volume 12, Issue 5, pp 363–365 | Cite as

Model Phase Separations of Proteins Using Aqueous/Ethanol Components

  • Ariel Louwrier


Of several saturated or partially saturated inorganic salt solutions forming stable biphasic systems when mixed with ethanol, K2HPO4/ethanol was used to partition several model proteins, including lysozyme, BSA, chymotrypsinogen and the DNA polymerase from Thermus aquaticus. Partitioning was pH-dependent, and was successfully used for protein isolation from a mixture. Varying degrees of enzymatic activities were maintained.


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Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Ariel Louwrier

There are no affiliations available

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