Model Phase Separations of Proteins Using Aqueous/Ethanol Components
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Of several saturated or partially saturated inorganic salt solutions forming stable biphasic systems when mixed with ethanol, K2HPO4/ethanol was used to partition several model proteins, including lysozyme, BSA, chymotrypsinogen and the DNA polymerase from Thermus aquaticus. Partitioning was pH-dependent, and was successfully used for protein isolation from a mixture. Varying degrees of enzymatic activities were maintained.
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