Skip to main content
SpringerLink
Log in

Solid-phase enzyme-linked assay for the screening of glycosidase activities and inhibitors

  • Published:
Biotechnology Techniques

Abstract

A solid-phase assay designed for the detection of glycosidase activity has been developed which detects 5 × 10–4 unit of α and β-galactosidases in 50 μL medium. The assay is highly stereo-specific. This assay is suitable for turbid or coloured fluids containing glycosidases and allows rapid and reliable Ki determination of glycosidase inhibitors. © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Dangelmaier, C.A. and Holmsen, H. (1980) Anal. Biochem. 104, 182-191.

    Google Scholar 

  • Edge, C.J., Rademacher, T.W., Wormald, M.R., Parekh, R.B., Butters, T.D., Wing, D.R. and Dwek, R.A. (1992) Proc. Natl. Acad. Sci. USA 89, 6338-6342.

    Google Scholar 

  • Fleet, G.W.J., Ramsden, N.G., Dwek, R.A., Rademacher, T.W. and Fellows, L.E. (1988) J. Chem. Soc., Chem. Comm. 483-485.

  • Friguet, B., Chaffotte, A.F., Djavadi-Ohaniance L. and Goldberg M.E. (1985) J. Immunol. Methods 77, 305-319.

    Google Scholar 

  • Gijsen, H.J.M., Qiao, L., Fitz, W. and Wong, C.-H. (1996) Chem. Rev. 96, 443-473.

    Google Scholar 

  • Keshvara, L.M., Newton, E.M., Good, A.H., Hindsgaul, O. and Palcic, M.M. (1992) Glycoconjugate J. 9, 16-20.

    Google Scholar 

  • Onodera, S. (1994) Biol. Pharm. Bull. 17, 29-33.

    Google Scholar 

  • Tawfik, D.S., Green, B.S., Chap, R., Sela, M. and Eshhar, Z. (1993) Proc. Natl. Acad. Sci. USA 90, 373-377.

    Google Scholar 

  • Winchester, B. and Fleet, G.W.J. (1992) Glycobiology 2, 199-210.

    Google Scholar 

  • Yeh, J.-C. and Cummings, R.D. (1996) Anal. Biochem. 236, 126-133.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UPRES n°2161 “;Biocatalyse”, Faculté des Sciences et des Techniques, 2, rue, de la Houssinière Nantes, France

    Thierry Dintinger, Jacques Ecorse, Arnaud Genre-Grandpierre, Yves Blériot & Charles Tellier

Authors
  1. Thierry Dintinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jacques Ecorse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arnaud Genre-Grandpierre
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yves Blériot
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Charles Tellier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dintinger, T., Ecorse, J., Genre-Grandpierre, A. et al. Solid-phase enzyme-linked assay for the screening of glycosidase activities and inhibitors. Biotechnology Techniques 12, 203–206 (1998). https://doi.org/10.1023/A:1008817307087

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008817307087

Keywords

Search

Navigation