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Letters in Peptide Science

, Volume 4, Issue 4–6, pp 359–364 | Cite as

Conformational studies of a benzodiazepine-like peptide in SDS micelles by circular dichroism, 1H NMR and molecular dynamics simulation

  • Marc Lecouvey
  • Céline Frochot
  • Laurent Miclo
  • Piotr Orlewski
  • Michel Marraud
  • Jean-Luc Gaillard
  • Manh Thong Cung
  • Régis Vanderesse
Article

Abstract

The conformation of a benzodiazepine-like decapeptide corresponding tothe YLGYLEQLLR fragment of a casein has been examined in a sodium dodecylsulfate micellar medium using circular dichroism, two-dimensional1H NMR spectroscopy and restrained molecular dynamicssimulation. The decapeptide adopts an amphipathic 310-helicoidstructure in which theE6···R10 ionic bridgestabilizes the C-terminus.

Benzodiazepines Circular dichroism GABAAreceptor Micellar medium Molecular dynamics 2D NMR 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Marc Lecouvey
    • 1
  • Céline Frochot
    • 1
  • Laurent Miclo
    • 2
  • Piotr Orlewski
    • 1
  • Michel Marraud
    • 1
  • Jean-Luc Gaillard
    • 2
  • Manh Thong Cung
    • 1
  • Régis Vanderesse
    • 1
  1. 1.LCPM, CNRS-URA 494, ENSIC-INPLNancy CedexFrance
  2. 2.LBSA, Unité associée à l'INRAUniversité Henri PoincaréVandoeuvre lès Nancy CedexFrance

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