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Biotechnology Techniques

, Volume 12, Issue 7, pp 553–556 | Cite as

Rapid method for the determination of lipid from the green alga Botryococcus braunii

  • Seog June Lee
  • Byung-Dae Yoon
  • Hee-Mock Oh
Article

Abstract

Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations. © Rapid Science Ltd. 1998

Keywords

Lipid Significant Relationship Lipid Content Green Alga Rapid Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Seog June Lee
  • Byung-Dae Yoon
  • Hee-Mock Oh

There are no affiliations available

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