World Journal of Microbiology and Biotechnology

, Volume 14, Issue 5, pp 685–688

Production, extraction and purificationof violacein: an antibiotic pigment producedby Chromobacterium violaceum

  • D. Rettori
  • N. Durán

DOI: 10.1023/A:1008809504504

Cite this article as:
Rettori, D. & Durán, N. World Journal of Microbiology and Biotechnology (1998) 14: 685. doi:10.1023/A:1008809504504


A procedure for the production, extraction, and purification of violacein was developed using Chromobacterium violaceum (CCT 3496) cultivated on cotton, in modified 1 litre Roux bottles. A surface tray bioreactor was built to perform these experiments. Violacein was extracted with commercial ethanol, and purified by filtration, Soxhlet extraction, crystallization and high performance liquid chromatography. The violacein was analysed and identified by proton and carbon-13 NMR spectroscopies, thermogravimetric analysis, mass spectrometry, UV-VIS spectroscopy and infrared spectroscopy. It was concluded that the product was highly purified violacein.

Antibiotic bioreactor Chromobacterium violaceum pigment violacein 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • D. Rettori
    • 1
  • N. Durán
    • 1
  1. 1.Instituto de Química, Laboratório de Química BiológicaUniversidade Estadual de CampinasBrazil

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