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Biotechnology Techniques

, Volume 12, Issue 3, pp 183–186 | Cite as

Analysis of Tween 80 as an esterase/ lipase substrate for lipolytic activity assay

  • Francisco J. Plou
  • Manuel Ferrer
  • Oscar M. Nuero
  • Maria V. Calvo
  • Miguel Alcalde
  • Fuensanta Reyes
  • Antonio Ballesteros
Article

Abstract

The Tween 80 assay to detect lipolytic activities in agar media was evaluated. A spectrophotometric assay for Tween 80 hydrolysis was established. The specific activities with Tween 80, as well as with some conventional lipase-type and esterase-type substrates, were measured using several lipases and esterases. The activity with Tween 80 was similar to that obtained with p-nitrophenyl butyrate; the enzyme activities with both substrates were between the esterase and lipase categories. © Rapid Science Ltd. 1998

Keywords

Enzyme Hydrolysis Enzyme Activity Agar Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Francisco J. Plou
    • 1
  • Manuel Ferrer
    • 1
  • Oscar M. Nuero
    • 1
  • Maria V. Calvo
    • 1
  • Miguel Alcalde
    • 1
  • Fuensanta Reyes
    • 2
  • Antonio Ballesteros
    • 1
  1. 1.Instituto de Catalisis, CSICCampus Universidad AutonomaMadridSpain
  2. 2.Centro de Investigaciones Biologicas, CSICMadridSpain

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