Abstract
The effects of 10, 50, 100, 200 and 300 mu g ml-1 of bromopropylate and methidathion were investigated in the cells of Azospirillum brasilense grown in chemically defined media and dialysed-soil media. The insecticide bromopropylate did not affect microbial growth, the levels of ATP and dinitrogen fixation of A. brasilense in either chemically defined media or dialysed-soil media. Methidathion significantly reduced dinitrogen fixation, the intracellular levels of ATP and growth in cells grown in chemically defined media. However, these negative effects were not significant in cells grown in dialysed-soil media, showing that A. brasilense can tolerate high concentrations (300 mu g ml-1) of methidathion under the present experiment conditions
Similar content being viewed by others
References
Balandreau, J. (1986) Ecological factors and adaptive processes in N2-fixing bacteria populations of the plant environment. Plant Soil 90, 73–93.
Bleakley, B.H., Gaskin, M.H., Hubbel, D.H. and Zam, S.G. (1988) Floc formation by Azospirillum lipoferum grown on poly-β-hydroxybutyrate. Appl. Environ. Microbiol. 54, 2986–95.
Chapman, A.G., Fall, L. and Atkinson, D.E. (1971) Adenylate energy charge in Escherichia coli during growth and starvation. J. Bacteriol. 108, 1072–86.
Dart, P.J. (1986) Nitrogen fixation associated with non-legumes in agriculture. Plant Soil 90, 303–34.
de la Rubia, T., Gonzalez-Lopez, J., Moreno, J., Martinez-Toledo, M.V. and Ramos-Cormenzana, A. (1987) Adenine nucleotide contents and energy charge of Azotobacter vinelandii grown at low phosphate concentrations. Arch. Microbiol. 147, 354–7.
Döbereiner, J. and Pedrosa, F.O. (1987) Nitrogen-fixing Bacteria in Nonleguminous Crops Plants. Madison: Sciencia Tech Publishers.
Gonzalez-Lopez, J. and Vela, G.R. (1981) True morphology of the Azotobacteraceae-filterable bacteria. Nature 289, 588–90.
Gonzalez-Lopez, J., Martinez-Toledo, M.V. and Salmeron, V. (1992) Effect of the insecticide methidathion on agricultural soil microflora. Biol. Fertil. Soils 13, 173–5.
Haahtela, K., Wartiovaara, T., Sundman, V. and Skujins, J. (1981) Root-associated N2 fixation (acetylene reduction) by Enterobacteriaceae and Azospirillum strains in cold-climate spodosols. Appl. Environ. Microbiol. 41, 203–6.
Lal, R. (1982) Accumulation, metabolism and effects of organophosphorus insecticides on microorganisms. Adv. Appl. Microbiol. 28, 149–200.
Martinez-Toledo, M.V., Gonzalez-Lopez, J., de la Rubia, T., Moreno, J. and Ramos-Cormenzana, A. (1988) Effect of inoculative with Azotobacter chroococcum on nitrogenase activity of Zea mays roots grown in agricultural soils under aseptic and non-sterile conditions. Biol. Fertil. Soils 6, 170–3.
Martinez-Toledo, M.V., Salmeron, V. and Gonzalez-Lopez, J. (1990) Effect of phenoxy and benzoic acid herbicides on nitrogenase activity and growth of Azospirillum brasilense. Soil Biol. Biochem. 22, 879–81.
Martinez-Toledo, M.V., Salmeron, V. and Gonzalez-Lopez, J. (1991) Effect of simazine on the biological activity of Azotobacter chroococcum. Soil Sci. 151, 459–67.
Okon, Y. and Itzigsohn, R. (1992) Poly-β-hydroxybutyrate metabolism in Azospirillum brasilense and the ecological role of PHB in the rhizosphere. FEMS Microbiol. Rev. 103, 131–40.
Omar, M.N.A., Berge, O., Hassanein, E.E. and Shalan, S.N. (1992) In vitro and in situ effects of herbicide thiobencarb on rice-Azospirillum association. Symbiosis 13, 55–63.
Pfaender, F.K. and Alexander, M. (1973) Effect of nutrient addition on the apparent cometabolism on DDT. J. Agricult. Food Chem. 21, 397–9.
Rouchaud, J., Metsue, M., van Himme, M., Gillet, J., Benoit, F. and Ceustermans, N. (1988) Soil metabolism of the herbicide chlorbromuron in rape celery crops. Soil Biol. Biochem. 20, 761–5.
Salmeron, V., Martinez-Toledo, M.V. and Gonzalez-Lopez, J. (1991) Effects of alachlor and metolachlor on the biological activity of Azospirillum brasilense grown in chemically defined and dialysed-soil media. Environ. Toxicol. Chem. 10, 493–9.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gomez, F., Salmeron, V., Rodelas, B. et al. Response of Azospirillum brasilense to the pesticides bromopropylate and methidathion on chemically defined media and dialysed-soil media. Ecotoxicology 7, 43–47 (1998). https://doi.org/10.1023/A:1008807701523
Issue Date:
DOI: https://doi.org/10.1023/A:1008807701523