European Journal of Plant Pathology

, Volume 106, Issue 2, pp 167–178 | Cite as

Evidence of the Presence of Two Serotypes of Rice Yellow Mottle Sobemovirus in Côte d'Ivoire

  • P. N'Guessan
  • A. Pinel
  • M.L. Caruana
  • R. Frutos
  • A. Sy
  • A. Ghesquière
  • D. Fargette
Article

Abstract

Serological variability of isolates of rice yellow mottle virus (RYMV) collected in Côte d'Ivoire was assessed by immunological tests with polyclonal and monoclonal antibodies (MAbs). Two serotypes (named S1 and S2) were distinguished. The S1 isolates had common epitopes which were absent in S2 isolates, whereas they lacked epitopes shared by S2 isolates. There was no evidence of S1 and S2 mixtures, although S1 and S2 isolates were sometimes found in nearby sites. Serotype S2 was more prevalent in Côte d'Ivoire than S1, and was in a large majority in the centre and the south of the country. By contrast, S1 occurred more widely in the north. S1 isolates were also found in neighbouring countries at the north of Côte d'Ivoire. In tests with monoclonal antibodies, three additional serotypes were found, one in West-Africa and two in East-Africa. Using the primers developed against an S2 isolate from Côte d'Ivoire, all S2 but not the S1 isolates were transcribed and amplified by RT-PCR, and another set of primers was developed to amplify S1 isolates. S1 and S2 have different biological properties, and competition between isolates of the two strains was apparent resulting in S2 dominance over S1. This was assessed using S1 and S2 strain specific MAbs, and it occurred whatever the pattern of inoculation or the rice variety tested. Differences in pathogenicity and virus titre did not account for strain competition, as there was no relation between symptom severity, virus content and serotype of the isolates in Oryza sativa indica cultivars.

Africa serotype virus diversity and distribution strain competition 

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References

  1. Al Moudallal Z, Briand JP and Van Regenmortel MHV (1982) Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 1: 1005–1010Google Scholar
  2. Awoderu VA (1991) Rice yellow mottle virus in West Africa. Tropical Pest Management 37: 356–362Google Scholar
  3. Awoderu VA, Alam MS, Thottapilly G and Alluri K (1987) Rice yellow mottle virus in upland rice. FAO Plant Protection Bulletin 35: 32–33Google Scholar
  4. Bakker W(1971) The new beetle vectors of rice yellow mottle in Kenya. Netherland Journal of Plant Pathology 77: 201–206Google Scholar
  5. Bakker W (1974) Characterisation and ecological aspects of rice yellow mottle virus in Kenya. Agricultural Research Reports, Wageningen 829: 1–152Google Scholar
  6. Ball EM (1990) Agar double diffusion plates (Ouchterlony): viruses. In: Hampton R, Ball E and De Boer S (eds) Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogens (pp 111–115) APS, St PaulGoogle Scholar
  7. Brugidou C, Holt C, Ngon A Yassi M, Zhang S, Beachy R and Fauquet C (1995) Synthesis of an infectious full-length cDNA clone of rice yellow mottle virus and mutagenesis of the coat protein. Virology 206: 108–115Google Scholar
  8. Clark MF and Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475–483Google Scholar
  9. Fauquet C and Thouvenel JC (1977) Isolation of the rice yellow mottle virus in Ivory Coast. Plant Disease Reporter 61: 443–446Google Scholar
  10. Halk ED and De Boer SH (1985) Monoclonal antibodies in plant-disease research. Annual Review of Phytopathology 23: 321–350Google Scholar
  11. Harlow E and Lane D (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, 726 ppGoogle Scholar
  12. Hull R (1988) The sobemovirus group. In: Koenig R (ed) The Plant Viruses. Polyedral Virions with Monopartite RNA Genomes, Vol 3 (pp 113–146) Plenum Press, New YorkGoogle Scholar
  13. John VT and Thottapilly G (1987) A scoring system for rice yellow mottle virus disease. International Rice Research Newsletter 12: 3–26Google Scholar
  14. John VT, Thottapilly G and Awoderu VA (1984) Occurrence of rice yellow mottle virus in some sahelian countries in West Africa. FAO Plant Protect Bulletin 32: 86–87Google Scholar
  15. Konate G, Traore O and Coulibaly M (1997) Characterisation of rice yellow mottle virus isolates in Sudano-Sahelian areas. Archives of Virology 142: 1117–1124Google Scholar
  16. Mansour AN and Baillis KW (1994) Serological relationships among rice yellow mottle virus isolates. Annals of Applied Biology 125: 133–140Google Scholar
  17. Matthews REF (1991) Plant Virology. Third Edition. Academic Press, Inc., 815 ppGoogle Scholar
  18. Ngon A Yassi M (1993) Caractérisation moléculaire du virus de la panachure jaune du riz (RYMV) et production de riz transgéniques. PhD of the Université des Sciences et Techniques du Languedoc Montpellier II, 143 ppGoogle Scholar
  19. Ngon A Yassi M, Ritzenthaler C, Brugidou C, Fauquet C and Beachy RN (1994) Nucleotide sequence and genome characterisation of rice yellow mottle virus RNA. Journal of General Virology 75: 249–257Google Scholar
  20. N'Guessan P (1999) Le virus de la panachure jaune du riz: diversité moléculaire et sérologique en Afrique et aspects épidémiologiques en Côte d'Ivoire. PhD de l'Ecole Nationale Supérieure Agronomique de MontpellierGoogle Scholar
  21. N'Guessan K, Sy A and Fargette D (1995) Caractérisation biologique et sérologique d'isolats du virus de la marbrure du riz (rice yellow mottle virus-RYMV) en Côte d'Ivoire. Proc. First Inter. Symposium on Rice Yellow Mottle Virus (RYMV), 18–22 September, 19 ppGoogle Scholar
  22. Raymundo SA and Buddenhagen IW (1976) A rice virus disease inWest Africa. International Rice Commission Newsletter 25: 58Google Scholar
  23. Raymundo SA and Konteh IK (1980) Distribution, screening methods and varietal reaction to rice pale yellowmottle disease. International Rice Commission Newsletter 29: 51–53Google Scholar
  24. Rochebrun M and Sablayrolles J (1993) Atlas du continent africain. Jeune Afrique Press, Paris, 175 ppGoogle Scholar
  25. Stocker JW, Foster HK and Miggiano V (1982) Generation of two new myeloma cell lines ‘PAI’ and ‘PAI-O’ for hybridoma production. Research Disclosure 217: 155–157Google Scholar
  26. Taylor DR, Fofie AS and Suma M (1990) Natural infection of rice yellow mottle virus disease (RYMV) on rice in Sierra Leone. International Rice Research Newsletter 15: 5–19Google Scholar
  27. Thomas JE, Massalski PR and Harrison BD (1986) Production of monoclonal antibodies to African cassava mosaic virus and differences in their reactivites with other whitefly-transmitted geminiviruses. Journal of General Virology 67: 2739–2748Google Scholar
  28. Urban LA, Sherwood JL, Rezende JAM and Melcher (1990) Examination of mechanisms of cross protection with non-transgenic plants. In: Fraser RSS (ed) Recognition and Response in Plant-Virus Interactions, NATO ASI series, Vol H 41 (pp 416–426) Springer-Verlag, Berlin, HeildelbergGoogle Scholar
  29. Van Regenmortel MHV (1982) Serology and Immunochemistry of Plant Viruses. Academic Press, New York, 302 ppGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • P. N'Guessan
    • 1
  • A. Pinel
    • 2
  • M.L. Caruana
    • 2
  • R. Frutos
    • 3
  • A. Sy
    • 4
  • A. Ghesquière
    • 5
  • D. Fargette
    • 6
  1. 1.CNRACôte d'Ivoire
  2. 2.LPRC, CIRAD/IRDMontpellier cedex 1France
  3. 3.IGEPAM, CIRADMontpellier cedex 1France
  4. 4.ADRAOCôte d'Ivoire
  5. 5.Genetrop, IRDMontpellier cedex 1France
  6. 6.LPRC, CIRAD/IRDMontpellier cedex 1France;

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