Advertisement

Genetic Resources and Crop Evolution

, Volume 47, Issue 2, pp 123–134 | Cite as

The Vigna angularis complex: Genetic variation and relationships revealed by RAPD analysis, and their implications for in situ conservation and domestication

  • Ru-Qiang Xu
  • Norihiko Tomooka
  • Duncan A. Vaughan
  • Koji Doi
Article

Abstract

The present study, using RAPD analysis, was undertaken to characterize genetic variation in three forms of V. angularis, cultivated, wild and weedy forms, and their relationships. The materials used consisted of 171 individuals (plants) or cultivars from 23 populations including 5 wild populations, 6 weedy populations, 6 cultivated populations and 6 populations with plants having wild and weedy or intermediate morphology, denoted here as complex populations. The materials used were collected on Honshu Island, Japan and seeds collected directly from the field were germinated for DNA extraction. In addition, 6 landrace accessions of V. angularis from the genebank were also analyzed. Genetic variation was highest in the wild form (Hg= 0.132; GD = 0.388), followed by the weedy form (Hg= 0.124; GD = 0.341) and the least in the cultivated form (Hg= 0.079; GD = 0.274). Intra-population genetic variation was high in the weedy and in the wild populations. However, inter-population was greater than intra-population genetic variation for all groups of populations studied in the V. angularis complex. 93% of the total diversity in the present study was exhibited by plants from complex populations and specific RAPD bands were found in these populations. Our results provide evidence that complex populations would be a logical focus for efforts to conserve the V. angularis complex in situ. Our results suggest that weedy populations are usually an ecotype of the wild form adapted to a different habitat.

domestication genetic variation in situ conservation RAPD V. angularis complex 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baudoin, J.P. & R. Maréchal, 1988. Taxonomy and evolution of the genus Vigna. In: Proceedings of the Second International Mungbean Symposium, pp. 2–12, Asia Vegetable Research and Development Center, Shanhua, Tainan, Taiwan.Google Scholar
  2. Beebe, S., Ch. Orlando Toro, A.V. Gonzalez, M.I. Chacon & D.G. Debouck, 1997. Wild-weed-crop complexes of common bean (Phaseolus vulgaris L., Fabaceae) in the Andes of Peru and Colombia, and their implications for conservation and breeding. Genet. Res. Crop Evol. 44: 73–91.Google Scholar
  3. Bonnin, I., T. Huguet, M. Gherardi, J.M. Prosperi & I. Olivieri, 1996. High level of polymorphism and spatial structure in a selfing plant species, Medicago truncatula (Leguminosae), shown using RAPD markers. Am. J. Bot. 83: 843–855.Google Scholar
  4. Cattan-Toupance, I., Y. Michalakis & C. Neema, 1998. Genetic structure of wild bean populations in their South-Andean centre of origin. Theor. Appl. Genet. 96: 844–851.Google Scholar
  5. Chalmers, K.J., R. Waugh, J.I. Sprent, A.J. Simons & W. Powell, 1992. Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers. Heredity 69: 465–472.Google Scholar
  6. Clausen, J.P., R.E. Keck & J.R. Hiesey, 1947. Heredity of geographically and ecologically isolated races. Am. Nat. 81: 114–133.Google Scholar
  7. Dawson, I.K., K.J. Chalmers, R. Waugh & W. Powell, 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD marker. Mol. Ecol. 3: 151–159.Google Scholar
  8. De Bustos, A., C. Casanova, C. Soler & N. Jouve, 1998. RAPD variation in wild populations of four species of the genus Hordeum (Poaceae). Theor. Appl. Genet. 96: 101–111.Google Scholar
  9. Gallois, A., J.C. Audran & M. Burrus, 1998. Assessment of genetic relationships and population discrimination among Fagus sylvatica L. by RAPD. Theor. Appl. Genet. 97: 211–219.Google Scholar
  10. Guarino, L., V. Rao Ramanatha & R. Reid (Eds.), 1995. Collecting plant genetic diversity. CAB International, Wallingford, U.K.Google Scholar
  11. Harlan, J.R., 1969. Evolutionary dynamics of plant domestication. Jap. J. Genet. 44 (suppl.): 337–343.Google Scholar
  12. Harlan, J.R., 1975. Crop and Man, pp. 85–104. American Society of Agronomy, Crop Science Society of America, Madison, WI, USA.Google Scholar
  13. Hymowitz, T., 1987. The grain legumes: an overview of crops and species. In: Grain legumes as alternative crops, pp. 32-44, Center for Alternative Crops and Products, University of Minnesota.Google Scholar
  14. Johns, T., Z. Huaman, C. Ochoa & P.E. Schmiediche, 1987. Relationships among wild, weed, and cultivated potatoes in the Solanum x anjuihuiri complex. Syst. Bot. 12: 541–552.Google Scholar
  15. Kresovich, S., J.G.K. Williams, J.R. McFerson, E.J. Routman & B.A. Schaal, 1992. Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor. Appl. Genet. 85: 190–196.Google Scholar
  16. Link, W., C. Dixkens, M. Singh, M. Schwall & A.E. Melchinger, 1995. Genetic diversity in European and Mediterranean faba bean germ plasm revealed by RAPD markers. Theor. Appl. Genet. 90: 27–32.Google Scholar
  17. Lumpkin, T.A. & D.C. McClary, 1994. Azuki bean: botany, production and uses. CAB International, Wallingford, U.K.Google Scholar
  18. Marshall, D.R. & A.H.D. Brown, 1975. Optimum sampling strategies in genetic conservation. In: O.H. Frankel & J.G. Hawkes (Eds.), Crop genetic resources for today and tomorrow, pp. 53–80, Cambridge University Press, Cambridge, London.Google Scholar
  19. Ohwi, J. & H. Ohashi, 1969. Azuki beans of Asia (in Japanese). Jap. J. Bot. 44: 29–31.Google Scholar
  20. Oka, H.I., 1988. Origin of cultivated rice, p.254, Japan Scientific Societies Press, TokyoGoogle Scholar
  21. Peet, R.K., 1974. The measurement of species diversity. Annu. Rev. Ecol. Syst. 5: 285–307.Google Scholar
  22. Pickersgill, B., 1971. Relationships between weedy and cultivated forms in some species of chili peppers (genus Capsicum). Evol. 25: 683–691.Google Scholar
  23. Rohlf, J.F., 1989. NTSYS. pc: numerical taxonomy and multivariate analysis system for the IBM pc microcomputer (and compatibles). Applied Biostatistics Inc, New York, N.Y.Google Scholar
  24. Saghai-Maroof, N.A., K.M. Soliman, R.A. Jorgensen & R.W. Allard, 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014–8018.Google Scholar
  25. Siriwardhane, D., Y. Egawa & N. Tomooka, 1991. Cross-compatibility of cultivated azuki bean (V. angularis) and rice bean (V. umbellata) with their wild relative. Plant Breeding 107: 320–325.Google Scholar
  26. Smartt, J., 1990. Grain legumes: evolution and genetic resources, p. 379, Cambridge University Press, Cambridge, U.K.Google Scholar
  27. Smartt, J. & T. Hymowitz, 1985. Domestication and evolution of grain legumes. In: Summerfield, R.J. & E.H. Roberts (Eds.), Grain legume crops, pp. 584–623, Collins Professional and Technical Books, London.Google Scholar
  28. Sonnante, G., T. Stockton, R.O. Nodari, V.L. Becerra Velasquez & P. Gepts, 1994. Evolution of genetic diversity during the domestication of common-bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 89: 629–635.Google Scholar
  29. Tateishi, Y.I., 1985. A revision of the azuki bean group, the subgenus Ceratotropis of the genus Vigna (Leguminosae), Ph. D. Dissertation, Tohoku University, Japan.Google Scholar
  30. Tateishi, Y. & H. Ohashi, 1990. Systematics of azuki bean group in the genus Vigna. In: K. Fujii, A.M.R Gatehouse, C.D. Johnson, R. Mitchel & T. Yoshida (Eds.), Bruchids and legumes: economics, ecology and coevolution, pp. 189–199, Kluwer Academic Publishers, Dordrecht, the Netherlands.Google Scholar
  31. Tomooka, N., D.A. Vaughan, R.Q. Xu & K. Doi, 1999. Wild relatives of crops conservation in Japan with a focus on Vigna spp.: Introduction. In: Annual Report on Exploration and Introduction of Plant Genetic Resources, Vol. 14, pp. 45–61, National Institute of Agrobiological Resources, Tsukuba, JapanGoogle Scholar
  32. Whitkus, R., M. de la Cruz & L. Mota-Bravo, 1998. Genetic diversity and relationships of cacao (Theobroma cacao L.) in southern Mexico. Theor. Appl. Genet. 96: 621–627.Google Scholar
  33. Yamaguchi, H., 1989. Weed azuki bean, an overlooked representative. Bull. Univ. Osaka Prefect. Ser. B 41: 21–27.Google Scholar
  34. Yamaguchi, H., 1990. A note on the distribution of semi-wild azuki beans in south-western Japan and their variation in seed color and size (in Japanese). Rep. Soc. Crop Sci. Breed. Kinki 35: 36–39.Google Scholar
  35. Yamaguchi, H., 1992. Wild and weed azuki beans in Japan. Econ. Bot. 46: 384–394.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Ru-Qiang Xu
    • 1
  • Norihiko Tomooka
    • 1
  • Duncan A. Vaughan
    • 2
  • Koji Doi
    • 1
  1. 1.Crop Evolutionary Dynamics Laboratory, Division of Genetic Resources IINational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan
  2. 2.Crop Evolutionary Dynamics Laboratory, Division of Genetic Resources IINational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan

Personalised recommendations