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European Journal of Plant Pathology

, Volume 105, Issue 3, pp 221–229 | Cite as

REMI (Restriction Enzyme Mediated Integration) and its Impact on the Isolation of Pathogenicity Genes in Fungi Attacking Plants

  • Regine Kahmann
  • Christoph Basse
Article

Abstract

Development of molecular techniques for phytopathogenic fungi aims at the identification of fungal genes whose products are essential for successful infection of the host plant. Initial approaches have relied on isolating candidate genes and generating null-mutations by homologous recombination. Unfortunately, the results of this strategy have not been overly successful. This has led to a search for alternatives which allow an unbiased identification of pathogenicity genes. One method, which has proved successful in several systems, is a tagging mutagenesis procedure termed restriction enzyme mediated integration (REMI). In this mini-review we describe this procedure and review its features and results of its use when applied to the identification of fungal genes required for disease development in planta.

mutagenesis transformation plant disease recombination plant pathogenic fungi 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Regine Kahmann
    • 1
  • Christoph Basse
    • 1
  1. 1.Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Str.1aMünchenGermany (E-mail

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