European Journal of Plant Pathology

, Volume 106, Issue 4, pp 329–337 | Cite as

Trichoderma harzianum T39 and T. virens DAR 74290 as Potential Biological Control Agents for Phytophthora erythroseptica

  • H.R. Etebarian
  • E.S. Scott
  • T.J. Wicks


Trichoderma harzianum isolate T39 and T. virens isolate DAR 74290 were evaluated as potential biological agents for control of pink rot of potato and root and stem rot of tomato caused by Phytophthora erythroseptica. Cell-free metabolites of T. virens DAR 74290 completely inhibited growth of P. erythroseptica in vitro and appeared to be fungicidal. T. virens DAR 74290 and Trichodex, a commercial formulation of T. harzianum T39, were tested for their ability to protect potato and tomato plants from disease caused by P. erythroseptica in glasshouse experiments. Trichodex and T. virens DAR 74290, alone and combined, reduced disease severity in shoots and roots of potatoes 10 weeks after inoculation with the pathogen. The yield of potatoes from plants treated with P. erythroseptica and T. virens DAR 74290 (mean of 12.9 g fresh weight/pot) was significantly greater than in controls inoculated with the pathogen alone (mean of 2.1 g/pot). Treatment with Trichodex alone increased the yield of tubers compared to the uninoculated controls. T. virens DAR 74290 increased the survival of tomato seedlings inoculated with the pathogen, and both this isolate and Trichodex decreased the severity of disease on tomato.

biocontrol pink rot potato disease tomato disease soilborne pathogen 


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  1. Adams PB (1990) The potential of mycoparasites for biological control of plant diseases. Annu Rev Phytopathol 28: 59–72Google Scholar
  2. Alexander BJR (1998) Studies on biological control of Phytophthora cactorum on apple. PhD thesis, University of Auckland, New ZealandGoogle Scholar
  3. Anonymous (1967) List of newdiseases in Iran. Iran J Plant Pathol 4: 37–40 (in Farsi, with English summary)Google Scholar
  4. Burgess DR and Hepworth G (1996) Biocontrol of sclerotinia stem rot (Sclerotinia minor) in sunflower by seed treatment with Gliocladium virens. Plant Pathol 45: 583–592Google Scholar
  5. Burgess DR and Keane P (1997) Biological control of Botrytis cinerea on chickpea seed with Trichoderma spp. and Gliocladium roseum: indigenous versus non-indigenous isolates. Plant Pathol 46: 910–918Google Scholar
  6. Carroll RB and Sasser M (1974) An outbreak of potato pink rot in Delaware. Plant Dis Reptr 58: 443–445Google Scholar
  7. Chet I (1987) Trichoderma-application, mode of action and potential as a biocontrol agent of soil-borne plant pathogenic fungi. In: Chet I (ed) Innovative Approaches to Plant Disease Control (pp 137–167) John Wiley and Sons, New York, USAGoogle Scholar
  8. Deacon JW (1991) Significance of ecology in the development of biocontrol agents against soilborne plant pathogens. Biocontrol Sci Technol 1: 5–20Google Scholar
  9. Dennis C and Webster J (1971a) Antagonistic properties of species groups of Trichoderma. I. Production of non-volatile antibiotics. Trans Brit mycol Soc 57: 25–39Google Scholar
  10. Dennis C and Webster J (1971b) Antagonistic properties of species groups of Trichoderma. III. Hyphal interactions. Trans Brit mycol Soc 57: 363–369Google Scholar
  11. Di Pietro A, Lorito M, Hayes CK, Broadway RM and Harman GE (1993) Endochitinase from Gliocladium virens: isolation, characterisation, and synergistic antifungal activity in combination with gliotoxin. Phytopathology 83: 308–313Google Scholar
  12. Dix JJ (1964) Colonisation and decay of bean roots. Trans Brit mycol Soc 47: 285–292Google Scholar
  13. Elad Y (1994) Biological control of grape grey mould by Trichoderma harzianum. Crop Prot 13: 35–38Google Scholar
  14. Elad Y, Chet I and Henis Y (1981) A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica 9: 59–67Google Scholar
  15. Elad Y, Katan J and Chet I (1980) Physical, biological and chemical control integrated for soilborne diseases in potatoes. Phytopathology 70: 418–422Google Scholar
  16. Ghisalberti EL and Sivasithamparam K (1991) Antifungal antibiotics produced by Trichoderma spp. Soil Biol Biochem 23: 1011–1020Google Scholar
  17. Gillings MR and Letham DB (1989) Phytophthora erythroseptica causing wilting and stunting of tomato. Aust Plant Pathol 18: 3–5Google Scholar
  18. Goodwin SB and McGrath MT (1995) Insensitivity to metalaxyl among isolates of Phytophthora erythroseptica causing pink rot of potato in New York. Plant Dis 79: 967Google Scholar
  19. Grisham MP, Taber RA and Barnes LW (1983) Phytophthora rot of potatoes in Texas caused by Phytophthora parasitica and P. cryptogea. Plant Dis 6: 1258–1261Google Scholar
  20. Harman GE, Latorre B, Agosin E, San Martin R, Riegel DG, Nielsen PA, Tronsmo A and Pearson RC (1996) Biological and integrated control of Botrytis bunch rot of grape using Trichoderma spp. Biol Control 7: 259–266Google Scholar
  21. Howell CR (1991) Biological control of Pythium damping-off of cotton with seed-coating preparation of Gliocladium virens. Phytopathology 81: 738–741Google Scholar
  22. Kay SJ and Stewart A (1994) Evaluation of fungal antagonists for control of onion white rot in soil box trials. Plant Pathol 43: 371–377Google Scholar
  23. Lennard JH (1980) Factors influencing the development of potato pink rot (Phytophthora erythroseptica). Plant Pathol 29: 80–86Google Scholar
  24. Lewis JA and Papavizas GC (1987) Reduction of inoculum of Rhizoctonia solani in soil by germlings of Trichoderma hamatum. Soil Biol Biochem 19: 195–201Google Scholar
  25. Little TM and Hills FJ (1978) Agricultural Experimentation Design and Analysis. John Wiley and Sons, New York, USAGoogle Scholar
  26. Lorito M, Hayes CK, Di Pietro A, Woo SL and Harman GE (1994) Purification, characterisation and synergistic activity of a glucan 1,3-?-glucosidase and an N-acetyl-?-glucosaminidase from Trichoderma harzianum. Phytopathology 84: 398–405Google Scholar
  27. Lumsden RD and Locke JC(1989) Biological control of damping-off caused by Pythium ultimum and Rhizoctonia solani with Gliocladium virens in soilless mix. Phytopathology 79: 361–366Google Scholar
  28. Lumsden RD, Ridout CJ, Vendemia ME, Harrison DJ, Waters RM and Walter JF (1992) Characterisation of major secondary metabolites produced in soilless mix by a formulated strain of the biocontrol fungus Gliocladium virens. Can J Microbiol 38: 1274–1280Google Scholar
  29. MacNish GC (1968) Pink rot of potatoes in Western Australia. Plant Dis Reptr 52: 280Google Scholar
  30. Mulrooney RP (1982) Evaluation of Ridomil for pink rot control. Fungicide Nematicide Tests 38: 105Google Scholar
  31. Na Lampang A (1994) Study on interaction between Sclerotium rolfsii Sacc. and selected antagonists. PhD thesis, The University of Adelaide, Australia.Google Scholar
  32. Nemec S, Datnoff LE and Strandberg J (1996) Efficacy of biocontrol agents in planting mixes to colonise plant roots and control root diseases of vegetables and citrus. Crop Prot 15: 735–742Google Scholar
  33. Ocana G and Tsao PH (1966) A selective medium for the direct isolation and enumeration of Phytophthora in soil. Phytopathology 56: 893Google Scholar
  34. Okhovat M, Hedjaroude GA, Rohani H and Zafari D (1994) Evaluation of antagonistic effect of few isolates of Trichoderma on Phytophthora erythroseptica, the cause of potato pink rot. Iran J Agric Sci 25: 61–72 (in Farsi, with English summary)Google Scholar
  35. O'Neill TM, Niv A, Elad Y and Shteinberg D (1996a) Biological control of Botrytis cinerea on tomato stem wounds with Trichoderma harzianum. Eur J Plant Pathol 102: 635–643Google Scholar
  36. O'Neill TM, Elad Y, Shteinberg D and Cohen A (1996b) Control of grapevine grey mould with Trichoderma harzianum T39. Biocontrol Sci Technol 6: 139–146Google Scholar
  37. Papavizas GC (1985) Trichoderma and Gliocladium: biology, ecology and potential for biocontrol. Annu Rev Phytopathol 23: 23–54Google Scholar
  38. Pethybridge GH (1913) On the rotting of potato tubers by a new species of Phytophthora having a method of sexual reproduction hitherto undescribed. Sci Proc Roy Dublin Soc (New Series) 13: 529–565Google Scholar
  39. Roiger J and Jeffers SN (1991) Evaluation of Trichoderma spp. for biological control of Phytophthora crown and root rot of apple seedlings. Phytopathology 81: 910–917Google Scholar
  40. Rowe RC and Nielsen LW (1981) Pink rot. In: Hooker WJ (ed) Compendium of Potato Diseases (pp 39–40) American Phytopathological Society, St Paul, Minnesota, USAGoogle Scholar
  41. Rowe RC and Schmitthenner AF (1977) Potato pink rot in Ohio caused by Phytophthora erythroseptica and P. cryptogea. Plant Dis Reptr 61: 807–810Google Scholar
  42. Samuels GJ (1996) Trichoderma: a review of biology and systematics of the genus. Mycol Res 100: 923–935Google Scholar
  43. Sivasithamparam K, Parker GA and Edwards CS (1979) Rhizosphere microorganisms of seminal and nodal roots of wheat grown in pots. Soil Biol Biochem 11: 155–160Google Scholar
  44. Smith VL, Wilcox WF and Harman GE (1990) Potential for biological control of Phytophthora root and crown rots of apple by Trichoderma and Gliocladium spp. Phytopathology 80: 880–885Google Scholar
  45. Stamps DJ (1978) Phytophthora erythroseptica. IMI descriptions of pathogenic bacteria and fungi. International Mycological Institute, Kew, England, No. 593Google Scholar
  46. Vargas LA and Nielsen LW (1972) Phytophthora erythroseptica in Peru: its identification and pathogenesis. Amer Potato J 49: 309–320Google Scholar
  47. Wicks TJ and Harding R (1996) Pink rot on potato. Potato Aust 7: 43Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • H.R. Etebarian
    • 1
  • E.S. Scott
    • 2
  • T.J. Wicks
    • 3
  1. 1.Abourayhan InstituteTehran UniversityTehranIran
  2. 2.Department of Applied and Molecular EcologyThe University of AdelaideGlen OsmondAustralia;
  3. 3.South Australian Research and Development InstituteAdelaideAustralia

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