Advertisement

European Journal of Plant Pathology

, Volume 104, Issue 6, pp 545–551 | Cite as

Formation of wall openings in root cells of Arabidopsis thaliana following infection by the plant-parasitic nematode Heterodera schachtii

  • Florian M.W. Grundler
  • Miroslaw Sobczak
  • Wladyslaw Golinowski
Article

Abstract

The induction and differentiation of feeding structures (syncytia) of the cyst nematode Heterodera schachtii in roots of Arabidopsis thaliana is accompanied by drastic cellular modifications. We investigated the formation of cell wall openings which occurred during syncytium differentiation. At the beginning of syncytium induction, a callose-like layer was deposited inside of the wall of the initial syncytial cell (ISC). First wall dissolutions developed by gradual widening of plasmodesmata between the ISC and neighbouring cells. As a general thickening of syncytial cell walls blocked existing plasmodesmata, other large openings were formed by enzymatic dissolution of intact walls by putative cellulase activity.

Arabidopsis thaliana cyst nematodes development histology syncytium ultrastructure 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Dangl JL (1993) The emergence of Arabidopsis thalianaas a model for plant-pathogen interactions. Adv Plant Pathol 10: 127-155Google Scholar
  2. Delmer DP (1987) Cellulose biosynthesis. Annu Rev Plant Physiol 38: 259-290Google Scholar
  3. Endo BY (1991) Ultrastructure of initial responses of susceptible and resistant soybean roots to infection by Heterodera glycines. Rev Nématol 14: 73-94Google Scholar
  4. Endo BY (1986) Histology and ultrastructural modification induced by cyst nematodes. In: Lamberti F and Taylor CE (eds) Cyst Nematodes. (pp 133-146) Plenum Press, New YorkGoogle Scholar
  5. Gipson I, Kim KS and Riggs RD (1971) An ultrastuctural study of syncytium development in soybean roots infected with Heterodera glycines. Phytopathology 61: 253-346Google Scholar
  6. Golinowski W, Grundler FMW and Sobczak M (1996) Changes in the structure of Arabidopsis thalianaduring female development of the plant-parasitic nematode Heterodera schachtii. Protoplasma 194: 103-116Google Scholar
  7. Grundler FMW (1989) Untersuchungen zur Geschlechtsdetermination des Rübenzystennematoden Heterodera schachtiiSchmidt. PhD thesis, University of KielGoogle Scholar
  8. Hussey RS, Mims CW and Westcott, SW (1992) Immunocytochemical localization of callose in root cortical cells parasitized by the ring nematode Criconemella xenoplax. Protoplasma 171: 1-6Google Scholar
  9. Jones MGK (1981) The development and function of plant cells modified by endoparasitic nematodes. In: Zuckerman BM and Rohde RA (eds) Plant parasitic nematodes. Vol III. (pp. 225-279) Academic Press, New YorkGoogle Scholar
  10. Jones MGK and Northcote DH (1972) Nematode-induced syncytium-a multinucleate transfer cell. J Cell Sci 10: 789-809PubMedGoogle Scholar
  11. Jones MGK and Payne HL (1977) Scanning electron microscopy of syncytia induced by Nacobbus aberransin tomato roots, and the possible role of plasmodesmata in their nutrition. J Cell Sci 23: 229-313Google Scholar
  12. Kobayashi I, Murdoch LJ, Kunoh H and Hardham AR (1995) Cell biology of early events in the plant resistance response to infection by pathogenic fungi. Can J Botany 73 (Suppl. 1): 418-425Google Scholar
  13. Kronestedt-Robards E and Robards AW (1991) Exocytosis in gland cells: In: Hawes CR, Coleman JOD and Evans DE (eds) Endocytosis, exocytosis and vesicle traffic in plants (pp. 199-232). Cambridge University Press, CambridgeGoogle Scholar
  14. Marchant R and Robards AW (1968) Membrane systems associated with the plasmalemma of plant cells. Ann Bot 32: 44-52Google Scholar
  15. Meyerowitz EM and Sommerville CR (1994) Arabidopsis. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  16. Nessler CL and Mahlberg PG (1981) Cytochemical localization of cellulase activity in articulated, anastomosing laticifers of Papaver somniferumL. (Papaveraceae). Am J Bot 68: 730-732Google Scholar
  17. Römpp H (1966) Chemie Lexikon. Franckh'sche Verlagshandlung, StuttgartGoogle Scholar
  18. Sexton R and Hall JL (1991) Enzyme cytochemistry. In: Hall JL and Hawes C (eds) Electron Microscopy of Plant Cells. (pp. 105- 180) Academic Press, LondonGoogle Scholar
  19. Sijmons PC, Grundler FMW, von Mende N, Burrows PR and Wyss U (1991) Arabidopsis thalianaas a new model host for plant-parasitic nematodes. Plant J 1: 245-254CrossRefGoogle Scholar
  20. Sobczak M (1996) Investigations on the structure of syncytia in roots of Arabidopsis thalianainduced by the beet cyst nematode Heterodera schachtiiand its relevance to the sex of the nematode. PhD thesis, University of KielGoogle Scholar
  21. Sobczak M, Grundler FMW and Golinowski W (1997) Changes in the structure of Arabidopsis thalianaroots induced during development of males of the plant parasitic nematode Heterodera schachtii. Europ J Plant Pathol 103: 113-124CrossRefGoogle Scholar
  22. Stender C, Lehmann H and Wyss U (1982) Feinstrukturelle Untersuchungen zur Entwicklung von Wurzel-Riesenzellen (Syncytien) induziert durch den Rübenzystennematoden Heterodera schachtii. Flora 172: 223-233Google Scholar
  23. Tanchak MA and Fowke LC (1987) The morphology of the multi-vesicular bodies in soybean protoplasts and their role in endocytosis. Protoplasma 138: 173-182Google Scholar
  24. Wyss U (1992) Observations on the feeding behaviour of Heterodera schachtiithroughout development, including events during moulting. Fundam. Appl Nematol 15: 75-89Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Florian M.W. Grundler
    • 1
  • Miroslaw Sobczak
    • 1
    • 2
  • Wladyslaw Golinowski
    • 2
  1. 1.Institut fuer Phytopathologie Universitaet KielKielGermany
  2. 2.Department of BotanyWarsaw Agricultural University, (SGGW)WarsawPoland

Personalised recommendations