Pharmacy World and Science

, Volume 20, Issue 5, pp 214–218 | Cite as

Cytogenetic effects on lymphocytes in osteoporotic patients on long-term fluoride therapy

  • Paul Asten
  • Firouz Darroudi
  • Adaqapalam T. Natarajan
  • Ido J. Terpstra
  • Symen A. Duursma


The genotoxicity of fluoride in vivo in seven patients with osteoporosis was cytogenetically investigated. The patients were treated with fluoride‐containing formulations (disodium monofluorophosphate and sodium fluoride) for a period of 15 months up to 49 months. Fluoride intake ranged from 22.6‐33.9 mg F-/day and serum fluoride concentrations were between 0.1 mg F-/l and 0.2 mg F-/l. Peripheral blood lymphocytes of these patients were cultured in vitro and examined for chromosomal aberrations, micronuclei in cytokinesis‐blocked binucleated lymphocytes as well as cell cycle progression. When a comparison was made between patients' group and a matched control group, it was found that fluoride at the tested concentrations had no detectable genotoxic potential in human lymphocytes in vivo.

Cytogenetic effects Lymphocytes Disodium monofluorophosphate Genotoxicity Osteoporosis Patient study Sodium fluoride 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Paul Asten
    • 1
  • Firouz Darroudi
    • 2
    • 3
  • Adaqapalam T. Natarajan
    • 2
    • 3
  • Ido J. Terpstra
    • 4
  • Symen A. Duursma
    • 5
  1. 1.Department of Hospital PharmacyUtrecht University HospitalUtrechtThe Netherlands
  2. 2.MGC, Department of Radiation Genetics and Chemical MutagenesisLeiden UniversityLeidenThe Netherlands
  3. 3.J.A. Cohen Institute of Radiopathology and Radiation ProtectionInteruniversity InstituteLeidenThe Netherlands
  4. 4.Yamanouchi Europe B.V.LeiderdorpThe Netherlands
  5. 5.Department of GeriatricsUtrecht University HospitalUtrechtThe Netherlands

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