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Pharmacy World and Science

, Volume 19, Issue 4, pp 197–201 | Cite as

Stability of nystatin in mouthrinses; effect of pH, temperature, concentration and colloidal silver addition, studied using an in vitro antifungal activity.

  • N. Vermerie
  • C. Malbrunot
  • M. Azar
  • P. Arnaud
Article

Abstract

Alkaline low concentration nystatin mouthrinses extemporanely prepared can be used to treat oropharyngeal candidiasis in immunodeficient patients. However, their expiration dates are not distinctly determined.

The stability of nystatin, added (as Mycostatine™) at a concentration of 14 400 U/ml in 10−4 N hydrochloric acid, purified water and 1.4% injectable sodium hydrogen carbonate with or without 0.002% colloidal silver (an antiseptic agent added because of its known antifungal potency) was studied after storage in tinted glass bottles at 5 °C and 22 °C over 11 days, and compared with reconstituted 100 000 U/ml aqueous Mycostatine™ oral suspension. At 2, 4, 7, 9, and 11 days after preparation. Samples were tested for pH, microbial contamination, and assayed by an in vitro microbiological test.

Neither significant variation of pH nor microbial contamination were in evidence. Nystatin 14 400 U/ml maintained at least 90% of its initial concentration for 4 days in acid at both temperatures, for 7 days (5 °C) and 4 days (22 °C) in aqueous and alkaline environments, for 9 days (5 °C) and 7 days (22 °C) in 1.4% injectable sodium hydrogen carbonate containing colloidal silver which showed an antifungal potency. The 100 000 U/ml aqueous Mycostatine™ oral suspension was stable for 9 days and 4 days at 5 °C and 22 °C respectively.

An ambulant patient can keep a low concentration alkaline antifungal mouthrinse at home for a week at 5 °C.

Antifungal mouthrinse Colloidal silver Drug concentration Drug stability Nystatin Sodium hydrogen carbonate 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • N. Vermerie
    • 1
  • C. Malbrunot
    • 2
  • M. Azar
    • 1
  • P. Arnaud
    • 1
  1. 1.Pharmacy ServiceJean Verdier Hospital AP-HPBondyFrance
  2. 2.Microbiology ServiceGilles de Corbeil HospitalCorbeil-EssonnesFrance

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