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Pharmacy World and Science

, Volume 21, Issue 1, pp 40–43 | Cite as

Detection of laxative abuse by urine analysis with HPLC and diode array detection

  • L.M.L. Stolk
  • K. Hoogtanders
Article

Abstract

A simple method is proposed for analysis of stimulant laxatives and metabolites of laxatives in urine. All stimulant laxatives commercially available in Germany, Begium and the Netherlands, the diphenylmethane derivatives and the anthraquinones, were included. Chromatography was performed with a standardized isocratic HPLC system with diode array detection ('STIP'), which is commonly used in the Netherlands for toxicological screening. The method was validated by ingestion of a normal dose of the laxatives by human volunteers. In all cases the expected laxative metabolite could be detected in urine twelve hours after intake. Also urine samples of patients, suspected of laxative abuse, were analyzed.

Laxative abuse Factitial diarrhea Chronic diarrhea Urine analysis Bisacodyl Bisoxatin Phenolphthalein Emodine Aloe‐emodine Rheine Danthron Picosulphate HPLC Diode array 

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References

  1. 1.
    Donowitz M. Magnesium-induced diarrhea and new insights into the pathobiology of diarrhea [editoral]. N Engl J Med 1991;324(15):1059-60.PubMedGoogle Scholar
  2. 2.
    Perkins SL, Livesey JF. A rapid high-performance thin-layer chromatographic urine screen for laxative abuse. Clin Biochem 1993;26(3):179-81.PubMedGoogle Scholar
  3. 3.
    De Wolff FA, de Haas EJM, Verwey M. A screening method for establishing laxatives abuse. Clin Chem 1981;27(6): 914-7.PubMedGoogle Scholar
  4. 4.
    Duncan A, Cameron A, Stewart MJ, Russell RI. Diagnosis of the abuse of magnesium and stimulant laxatives. Ann Clin Biochem 1991;28(6):568-73.PubMedGoogle Scholar
  5. 5.
    Morton J. The detection of laxative abuse. Ann Clin Biochem 1987;24(1):107-8.PubMedGoogle Scholar
  6. 6.
    Kok RM, Faber DB. Qualitative and quantitative analysis of some synthetic chemically acting laxatives in urine by gaschromatography-mass spectrometry. J Chromatogr 1981; 222:389-98.PubMedGoogle Scholar
  7. 7.
    Fullinfaw RO, Bury RW, Moulds RFW. Screening procedure for stimulant laxatives in urine using high-performance liquid chromatography with diode array detection. J Chromatogr 1988;433:131-40.PubMedGoogle Scholar
  8. 8.
    Phillips S, Donaldson L, Geisler K, Pera A, Kochar R. Stool composition in factitial diarrhea: a 6-year experience with stool analysis. Ann Intern Med 1995;123:97-100.PubMedGoogle Scholar
  9. 9.
    Snoeren RA, Duchateau AMJA, Conemans JMH, Gerkens FDA, Pijnenburg CC, Barella CGJ, et al. Systematic toxicological analysis of drugs with HPLC and diode array detection. (Abstract) Pharm Weekbl (Sci) 1989;11(3):E16.Google Scholar
  10. 10.
    Pijnenburg CC, Duchateau AMJA, Conemans JMH, Gerkens FDA, Snoeren RA, Barella CGJ, et al. Systematische toxicologische analyse van geneesmiddelen met behulp van HPLC en UV-detectie. Ziekenhuisfarmacie 1990;6(1):1-4.Google Scholar
  11. 11.
    Jauch R, Hankwitz R, Beschke K, Pelzer H. Bis-(p-hydroxyphenyl)-pyridyl-2-methane: the common laxative principle of bisacodyl and sodium picosulfate. Arzneim Forsch 1975;25(11):1796-800.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • L.M.L. Stolk
  • K. Hoogtanders

There are no affiliations available

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