Genetic Resources and Crop Evolution

, Volume 45, Issue 6, pp 533–539 | Cite as

Incongruence between RFLPs of chloroplast DNA and morphological classification in east Asian pear (Pyrus spp.)

  • Hiroyuki Iketani
  • Tohru Manabe
  • Nagao Matsuta
  • Tomoya Akihama
  • Tateki Hayashi
Article

Abstract

Polymorphism of chloroplast DNA of 106 accessions of Pyrus (mainly east Asian accessions) was examined. Four haplotypes were observed with the combination of three independent restriction site mutations digested by EcoO109 I, Sal I, and Xba I, respectively. In the occidental pear accessions only the most plesiomorphic type was observed, whereas all four types appeared in the oriental pear accessions. This suggests that the oriental species of Pyrus and occidental ones may have evolved independently. The distribution of four haplotypes in the east Asian pear was quite incongruent with the species or infrageneric classification using mainly morphological characters. Considering the high crossability and much occurrence of suspected interspecific hybrids in wild populations, this disaccord is inferred to be the results of the hybridization and introgression between species.

chloroplast DNA hybridization introgression Pyrus RFLP 

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References

  1. Arnold, M.N., C.M. Buckner & J.J. Robinson, 1991. Pollenmediated introgression and hybrid speciation in Louisiana irises. Proc. Natl. Acad. Sci. USA 88: 1398-1402.Google Scholar
  2. Bailey, L.H., 1916. Pyrus. In: Standard Cyclopedia of Horticulture vol. V, pp. 2865-2878, Macmillan, New York.Google Scholar
  3. Challice, J. & M.N. Westwood, 1973. Numerical taxonomic studies of the genus Pyrus using both chemical and botanical characters. Bot. J. Linn. Soc. 67: 121-148.Google Scholar
  4. Dally, A.M. & G. Second, 1990. Chloroplast DNA diversity in wild and cultivated species of rice (Genus Oryza, section Oryza). Cladistic-mutation and genetic-distance analysis. Theor. Appl. Genet. 80: 209-222.Google Scholar
  5. Doebly, J., R. von Bothmer & S. Larson, 1992. Chloroplast DNA variation and the phylogeny of Hordeum (Poaceae). Amer. J. Bot. 79: 576-584.Google Scholar
  6. Doyle, J.J., J.L. Doyle & A.H.D. Brown, 1990a. Chloroplast-DNA polymorphism and phylogeny in the B genome of Glycine subgenus Glycine (Leguminosae). Amer. J. Bot. 77: 772-782.Google Scholar
  7. Doyle, J.J., J.L. Doyle & A.H.D. Brown, 1990b. A chloroplast-DNA phylogeny of the wild perennial relatives of soybean (Glycine subgenus Glycine): congruence with morphological and crossing groups. Evolution 44: 371-389.Google Scholar
  8. Iketani, H., 1994. Chloroplast DNA diversity in Pyrus and related genera. Gamma field symposia (Institute of Radiation Breeding, NIAR, MAFF, Japan) 32: 63-69.Google Scholar
  9. Kajiura, I. & S. Suzuki, 1980. Variation in fruit shapes of Japanese pear cultivars (Pyrus serotina Rehder var. culta Rehder); geographic differentiations and changes by breeding. Jpn. J. Breed. 30: 309-328 (in Japanese).Google Scholar
  10. Kajiura, I., S. Yamaki, M. Omura, T. Akihama & Y. Machida, 1979. Improvement of sugar content and composition in fruits, and classification of east Asian pears by the principal component analysis of sugar composition in fruits. Jpn. J. Breed. 29: 1-12.Google Scholar
  11. Kikuchi, A., 1948. Horticulture of Fruit Trees, vol 1. Yokendo, Tokyo (in Japanese).Google Scholar
  12. Kitamura, S., 1979. Pyrus. In: Kitamura, S. & G. Murata (Eds), Coloured Illustrations of Woody Plants of Japan, vol II, pp. 42- 47, Hoikusha, Osaka, (in Japanese).Google Scholar
  13. Koehne, E., 1890. Die Gattung der Pomaceen. Wissenshaftliche Beitrage zum Programm des Falk-Realgymnasium zu Berlin, Nr. 95: 1-33, Taf. 1-2.Google Scholar
  14. Lee, S.H., 1948. A Taxonomic survey of the oriental pears. Proc. Amer Soc. Hort. Sci. 51: 152-156.Google Scholar
  15. National Institute of Fruit Tree Science, 1997. List of genetic resources of fruit trees.Google Scholar
  16. Phipps, J.B., K.R. Robertson, J.R. Rohrer, P.G. Smith, 1991. Origins and evolution of subfam. Maloideae (Rosaceae). Syst. Bot. 16: 303-332.Google Scholar
  17. Rieseling, L.H. & S.J. Brunsfeld, 1992. Molecular evidence and plant introgression. In: Soltis, P.S., D.S. Soltis & J.J. Doyle (Eds), Molecular Systematics, pp. 151-176, Chapman and Hill, New York.Google Scholar
  18. Rieseling, L.H., D.E. Soltis & J.D. Palmer, 1988. A molecular reexamination of introgression between Helianthus annuus and H. bolanderi (Compositae). Evolution 42: 227-238.Google Scholar
  19. Rogers, S.O. & A.J. Bendich, 1988. Extraction of DNA from plant tissues. In: Gelvin, S.B., R.A. Schilperoort & D.S. Verma (Eds), Plant Molecular Biology Manual, sect A/6, pp. 1-10, Kluwer Academic Publ., Dordrecht.Google Scholar
  20. Rubtsov, G.A., 1944. Geographical distribution of the genus Pyrus and trends and factors in its evolution. Amer. Nat. 78: 358-366.Google Scholar
  21. Soltis, D.E., P.S. Soltis & B.G. Milligan, 1992. Intraspecific chloroplast DNA variation: systematic and phylogenetic implications. In: Soltis, D.E., P.S. Soltis & J.J. Doyle (Eds), Molecular Systematics of Plants, pp. 117-150, Chapman and Hall, New York.Google Scholar
  22. Terpo, A., J. do Amiral Franco, 1968. Pyrus. In: Tutin, T.G., V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Walters & D.A. Webb (Eds), Flora Europaea vol. II, pp. 65-66, Cambridge University Press, Cambridge.Google Scholar
  23. van Ham, R.C.H.J., T.H.M. Mes, H. 'tHart & J.M. Sandbrink, 1992. Clone bank and physical map of Sedum album (Crassulaceae) chloroplast DNA. Biochem. Syst. Ecol. 20: 243-253.Google Scholar
  24. Whittmore, A.T. & B.A. Schall, 1990. Interspecific gene flow in sympatric oaks. Proc. Natl. Acad. Sci. USA 88: 2540-2544.Google Scholar
  25. Wiley, E.O., 1981. Phylogenetics, John Wiley and Sons, New York.Google Scholar
  26. Yü, T.T. (ed.), 1979. Taxonomy of the fruit tree in China, Agriculture Press, Beijing (in Chinese).Google Scholar
  27. Yü, T.T. & Ku, T.C., 1974. Pyrus. In: Yü, T.T. (Ed), Flora Reipublicae Popularis Sinicae, vol 36, pp. 354-372, Science Press, Beijing (in Chinese).Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Hiroyuki Iketani
    • 1
  • Tohru Manabe
    • 1
    • 2
  • Nagao Matsuta
    • 1
  • Tomoya Akihama
    • 2
  • Tateki Hayashi
    • 1
  1. 1.Ministry of Agriculture, Forestry and Fisheries, 2-1National Institute of Fruit Tree ScienceFujimoto, TsukubaJapan
  2. 2.Faculty of AgricultureMeiji University, 1-1-1Higashi-mita, Tama-ku, Kawasaki, KanagawaJapan

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