Abstract
Resistance tests were made on seedlings of transformed lines of Nicotiana benthamiana which contain a transgene encoding the coat protein (CP) gene of a Scottish isolate of potato mop-top virus (PMTV). This transgene has been reported to confer strong resistance to the PMTV isolate from which the transgene sequence was derived and also to a second Scottish isolate. Plants of lines of the transgenic N. benthamiana were as resistant to two Swedish and two Danish PMTV isolates as to a Scottish isolate, and of five lines tested, greater than 93.5% of transgenic plants were immune. The coat protein gene sequences of these four Scandinavian isolates were very similar to those of the two Scottish isolates. The greatest divergence between the isolates was three amino acid changes and there was less than 2% change in CP gene nucleotide sequence. It is concluded that the PMTV CP transgene used in these experiments could confer resistance against isolates from different geographical areas because it is becoming apparent that the CP genes of PMTV isolates are highly conserved.
Similar content being viewed by others
References
Acosta O, Barker H and Mayo MA (1994) Prospects for improving virus resistance of potato crops in Colombia by transgenic technology. Fitopatologia Colombiana 18: 66-77
Anderson EJ, Stark DM, Nelson RS, Powell PA, Tumer NE and Beachy RN (1989) Transgenic plants that express the coat protein genes of tobacco mosaic virus or alfalfa mosaic virus interfere with disease development of some nonrelated viruses. Phytopathology 79: 1284-1290
Arif M, Torrance L and Reavy B (1994) Improved efficiency of detection of potato mop-top furovirus in potato tubers and in the roots and leaves of soil bait plants. Potato Research 37: 373-381
Arif M, Torrance L and Reavy B (1995) Acquisition and transmission of potato mop-top furovirus by a culture of Spongospora subterranea f. sp. subterranea derived from a single cystosorus. Ann Appl Biol 126: 493-503
Barker H (1995) Host genes and transgenes that confer resistance to a Scotish isolate of potato leafroll virus are also effective against a Peruvian isolate. Potato Research 38: 283-288
Bevan M, Barnes WM and Chilton MD (1983) Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Research 11: 369-385
Devereaux J, Haeberli P and Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12: 378-395
Harrison BD and Jones RAC (1971) Factors affecting the development of spraing in potato tubers infected with potato mop-top virus. Ann Appl Biol 68: 281-289
Jones RAC and Harrison BD (1969) The behaviour of potato moptop virus in soil and evidence for its transmission by Spongospora subterranea (Wallr.) Lagerh. Ann Appl Biol 63: 1-17
Kashiwazaki S, Scott KP, Reavy B and Harrison BD(1995) Sequence analysis and gene content of potato mop-top virus RNA 3: Further evidence of heterogeneity in the genome organization of furoviruses. Virology 206: 701-706
Lomonossoff GP (1995) Pathogen-derived resistance to plant viruses. Ann Review Phytopath 33: 323-343
Mayo MA, Torrance LT, Cowan G, Jolly CA, Macintosh SM, Orrega R, Barrera C and Salazar LF (1996) Conservation of coat protein sequence among isolates of potato mop-top virus from Scotland and Peru. Arch. Virol. 141: 1115-1121
Nejidat A and Beachy RN(1990) Transgenic tobacco plants expressing a tobacco mosaic virus coat protein gene are resistant to some tobamoviruses. Mol Plant-Microbe Interact 3: 247-251
Nielsen SL and Engsbro B (1992) Susceptibility of potato cultivars to spraing caused by primary infection of tobacco rattle virus and potato mop-top virus. Tidsskr Planteavl 96: 507-516
Reavy B, Arif M, Kashiwazaki S, Webster KD and Barker H (1995) Coat protein-mediated immunity to potato mop-top virus in Nicotiana benthamiana is effective against fungal inoculation. Mol Plant-Microbe Interact 8:286-291
Sandgren M (1995) Potato mop-top virus (PMTV): distribution in Sweden, development of symptoms during storage and cultivar trials in field and glasshouse. Potato Research 38: 387-397
Stark DM and Beachy RN (1989) Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7: 1257-1262
Torrance L, Cowan GH and Pereira LG (1993) Monoclonal antibodies specific for potato mop-top virus, and some properties of the coat protein. Ann Appl Biol 122: 311-322
Tumer NE, Kaniewski W, Haley L, Gehrke L, Lodge JK and Sanders P (1991) The second amino acid of alfalfa mosaic virus coat protein is critical for coat protein-mediated protection. Proc Nat Acad Sci USA 88: 2331-2335
Van Dun CMP and Bol JF (1988) Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167: 649-652
Verwoerd TC, Dekker BMM and Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17: 2362
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reavy, B., Sandgren, M., Barker, H. et al. A coat protein transgene from a Scottish isolate of potato mop-top virus mediates strong resistance against Scandinavian isolates which have similar coat protein genes. European Journal of Plant Pathology 103, 829–834 (1997). https://doi.org/10.1023/A:1008645504867
Issue Date:
DOI: https://doi.org/10.1023/A:1008645504867