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Pharmacy World and Science

, Volume 19, Issue 5, pp 246–250 | Cite as

Discussion of the AED workshop.

  • Zafar Iqbal
  • J.M. Midgley
  • D.G. Watson
  • S.D. Karditsas
  • G.N. Dutton
  • W. Wilson
Article

Abstract

A method has been developed for the determination of the molecular weight of hyaluronic acid by high performance gel permeation chromatography. The molecular weight distribution was determined using three polymeric columns. Columns were calibrated using Pullulan Polymer molecular weight standards. The average molecular weight (± SD) of the hyaluronic acid in bovine vitreous humour and Healon® were 1.20 ¥ 106 Da (± 0.49 ¥ 106) and 3.01 ¥ 106 Da (± 0.14 ¥ 106), respectively. Furthermore, the depolymerization of the hyaluronic acid in the presence of ascorbic acid (the concentration found in human eye) was studied. Samples incubated in the presence of atmospheric air showed more depolymerization than those where atmospheric air had been flushed-out with nitrogen.

Ascorbic acid Depolymerization Eye High performance gel permeation chromatography Hyaluronic acid Molecular weight 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Zafar Iqbal
    • 1
  • J.M. Midgley
    • 1
  • D.G. Watson
    • 1
  • S.D. Karditsas
    • 2
  • G.N. Dutton
    • 2
  • W. Wilson
    • 1
  1. 1.Department of PharmacyUniversity of StrathclydeGlasgowU.K
  2. 2.Tennent Institute of Ophthalmology, Western InfirmaryGlasgowU.K

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