Genetic Resources and Crop Evolution

, Volume 45, Issue 6, pp 561–564 | Cite as

A study on glutamate oxaloacetate transaminase isozymes of citron cultivars

  • E. Protopapadakis
  • X. Papanikolaou


For the purposes of experimental taxonomy, glutamate oxaloacetate transaminase (GOT) (EC isozymes have been studied. The zymograms of glutamate oxaloacetate transaminase indicated the existence of three genotypes. In addition to the two loci of GOT reported (Torres et al., 1978b) a new locus was detected. Three loci composed of six alleles have been identified after PAGE gel electrophoresis of pollen extracts belonging to 7 cultivars. Two of the cultivars, 'Fimatiodes' and 'Roxani', present a genetic variation of the other five cultivars of Citrus medica L., which may indicate perhaps a separate taxonomic position.

Citrus medica Cultivar isozyme pollen 


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  1. Cardy, B.J., C.W. Stuber & M.M. Goodman, 1980. Techniques for starch gel electrophoresis of enzyme from maize (Zea mays L.). Institute of Statistic Mimeograph Series No. 1317, December 1980. North Carolina State University.Google Scholar
  2. Duran-Vila, N., J.A. Pina, J.F. Ballester, J. Juarez, C.N. Roistacher, R. Rivera-Bustamante & J.S. Semancik, 1988. The citrus exocortis disease: a complex of viroid-RNAs. Proceedings of the International Organization of Citrus virologists 10, 152-164, Riverside.Google Scholar
  3. Grant, V., 1971. Plant speciation. Columbia University Press, New York.Google Scholar
  4. Hirai, M., I. Kozaki & I. Kajiura, 1986. Isozyme analysis and phylogenic relationship of citrus. Japan. J. Breed. 36: 377-389.Google Scholar
  5. Hirai, M. & I. Kajiura, 1987. Genetic analysis of leaf isozymes in citrus. Japan. J. Breed. 37: 377-388.Google Scholar
  6. Mäkinen, Y. & T. MacDonald, 1968. Isoenzyme polymorphisms in flowering plants. II. Pollen enzymes and isoenzymes. Physiol. Plant. 21: 477-486.Google Scholar
  7. O'Farrel, P., 1975. High resolution two dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007-4021.Google Scholar
  8. Protopapadakis, E.E., 1987. Identification by isoenzymes of five cultivars of Citrus medica grafted on four rootstocks. J. Hort. Sci. 62 (3): 413-419.Google Scholar
  9. Protopapadakis, E., 1995. Genetic analysis of glutamate oxalacetate transaminase from leaves of Cretan tulip species. J. Hort. Sci 70 (6): 919-923.Google Scholar
  10. Rahman, M.M. & N. Nobumasa, 1994. Use of glutamateoxaloacetate transaminase isozymes for detection of hybrids among genera of the ‘true citrus fruit trees’. Sci. Horticult. 58: 197-206.Google Scholar
  11. Roose, M.L., 1988. Isozymes and DNA restriction fragment length in Citrus breeding and systematics. In: R. Goren & K. Mendel (Eds) Proc. Sixth Int. Citrus Congr., Vol. I, pp. 155-165.Google Scholar
  12. Scora, R.W., 1975. On the history and origin of Citrus. Bull. Torrey Club 102: 369-375.Google Scholar
  13. Shaw, C. & R. Pradas, 1970. Starch gel electrophoresis of enzymes - A compilation of recipes. Biochem Genet 4: 297-320.Google Scholar
  14. Simpson, R.B. & W. Kauzman, 1953. The kinetics of protein denaturation of the optical rotation of ovalbumin in urea solutions. J. Am. Chem. Soc. 75: 5139-5152.Google Scholar
  15. Soost, R.K. & A.M. Torres, 1981. Leaf isozymes as genetic markers in citrus. Proc. Int. Soc. Citricult. 1: 7-10.Google Scholar
  16. Tolkowsky, S., 1938. Hesperides: a history of the culture and use of citrus fruits. John Bale & Curnow, London.Google Scholar
  17. Torres, A.M., U. Diedenhoffen, B.O. Bergh & R.J. Knight, 1978a. Enzyme polymorphisms as genetic markers in the avocado. Amer. J. Bot. 65: 134-139.Google Scholar
  18. Torres, A.M., R.K. Soost & U. Diedenhofen, 1978b. Leaf isozymes as genetic markers in citrus. Amer. J. Bot. 65: 869-881.Google Scholar
  19. Torres, A.M., T. Mau-Lastovicka, T.E. Williams & R.K. Soost, 1985. Segregation, distortion and linkage of Citrus and Poncirus isozymes genes. J. Hered. 76: 289-294.Google Scholar
  20. Zhong, G., 1993. A study on glutamate oxaloacetate transaminase isozymes of citrus germplasm resources. Acta Phytotaxon. Sin. 31 (2): 162-169.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • E. Protopapadakis
    • 1
  • X. Papanikolaou
    • 2
  1. 1.Institute of Subtropical Plants and Olive TreeChaniaGreece
  2. 2.Agricultural Research Station of RhodesGreece

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