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Journal of Biomolecular NMR

, Volume 15, Issue 2, pp 177–180 | Cite as

An efficient strategy for assignment of cross-peaks in 3D heteronuclear NOESY experiments

  • Tammo Diercks
  • Murray Coles
  • Horst Kessler
Article

Abstract

The question is addressed of how maximal structural NOE data on double labelled proteins can be acquired with a minimal set of NOESY experiments. Two 3D-NOESY spectra are reported which, in concert with other commonly used spectra, provide a convenient strategy for NOE assignment. The 3D CNH-NOESY and 3D NCH-NOESY provide NOE connectivities between amide protons and carbon-bound protons and constitute orthogonal heteronuclear filters which eliminate diagonal signals, considerably improving spectral quality. Two different heteronuclear chemical shift dimensions are recorded in the spectra, thus exploiting the extra dispersion of the heteronucleus and considerably simplifying assignment.

CNH-NOESY 3D-NOESY heteronuclear NMR NCH-NOESY orthogonal filtering 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Tammo Diercks
    • 1
  • Murray Coles
    • 1
  • Horst Kessler
    • 1
  1. 1.Institut für Organische Chemie und BiochemieTechnische Universität München

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