Journal of Biomolecular NMR

, Volume 17, Issue 2, pp 99–109 | Cite as

A new method for the simultaneous measurement of magnitude and sign of 1DCH and 1DHH dipolar couplings in methylene groups

  • Teresa Carlomagno
  • Wolfgang Peti
  • Christian Griesinger
Article

Abstract

Heteronuclear dipolar couplings of the protein backbone have proven to have a big impact on the accuracy of protein NMR structures. H,H dipolar couplings might have the same impact on side chains. Here we present a method that combines both heteronuclear and homonuclear dipolar couplings to investigate the local conformation of methylene groups. A new pulse sequence (SPITZE-HSQC) is presented, that allows to measure the two C,H and the H,H dipolar couplings at the same time, using spin state selective transfers. The new method has been applied to the methylene groups of glycines in the protein ubiquitin. The C,H and the H,H dipolar couplings might have a key role in fast stereospecific assignment of protons in CH2 groups.

dipolar couplings methylene groups spin state selective transfer structure determination SPITZE-HSQC ubiquitin 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Teresa Carlomagno
    • 1
  • Wolfgang Peti
    • 1
  • Christian Griesinger
    • 1
  1. 1.Institut für Organische ChemieUniversität FrankfurtFrankfurtGermany

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