Journal of Biomolecular NMR

, Volume 14, Issue 3, pp 277–280

Simultaneous determination of Ψ and Φ angles in proteins from measurements of cross-correlated relaxation effects

  • Philippe Pelupessy
  • Elisabetta Chiarparin
  • Ranajeet Ghose
  • Geoffrey Bodenhausen
Article

Abstract

A method is presented to determine both φ and ψ backbone angles in proteins simultaneously. This is achieved by measuring the effect on two-spin coherences of cross-correlation between 15 N-1HN and 13\({}^{{\text{13}}}{\text{C}}^\alpha{\text{ - }}{}^{\text{1}}{\text{H}}^\alpha\) vectors. The cross-correlation rates are obtained by comparing two complementary three-dimensional experiments.

cross-correlation dihedral angles protein structure 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Philippe Pelupessy
    • 1
  • Elisabetta Chiarparin
    • 1
  • Ranajeet Ghose
    • 1
  • Geoffrey Bodenhausen
    • 1
  1. 1.Section de ChimieUniversité de Lausanne, BCHLausanneSwitzerland

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