, Volume 10, Issue 2, pp 93–104

Biodegradation of 2-methyl, 2-ethyl, and 2-hydroxypyridine by an Arthrobacter sp. isolated from subsurface sediment

  • Edward J. O'Loughlin
  • Gerald K. Sims
  • Samuel J. Traina


A bacterium capable of degrading 2-methylpyridine was isolated by enrichment techniques from subsurface sediments collected from an aquifer located at an industrial site that had been contaminated with pyridine and pyridine derivatives. The isolate, identified as an Arthrobacter sp., was capable of utilizing 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine as primary C, N, and energy sources. The isolate was also able to utilize 2-, 3-, and 4-hydroxybenzoate, gentisic acid, protocatechuic acid and catechol, suggesting that it possesses a number of enzymatic pathways for the degradation of aromatic compounds. Degradation of 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine was accompanied by growth of the isolate and release of ammonium into the medium. Degradation of 2-methylpyridine was accompanied by overproduction of riboflavin. A soluble blue pigment was produced by the isolate during the degradation of 2-hydroxypyridine, and may be related to the diazadiphenoquinones reportedly produced by other Arthrobacter spp. when grown on 2-hydroxypyridine. When provided with 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine simultaneously, 2-hydroxypyridine was rapidly and preferentially degraded; however there was no apparent biodegradation of either 2-methylpyridine or 2-ethylpyridine until after a seven day lag. The data suggest that there are differences between the pathway for 2-hydroxypyridine degradation and the pathway(s) for 2-methylpyridine and 2-ethylpyridine.


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  1. Aislabie J, Rothenburger S & Atlas RM (1989) Isolation of microorganisms capable of degrading isoquinoline under earobic conditions. Appl. Environ. Microbiol. 55: 3247–3249.Google Scholar
  2. Bennett JL, Updegraff DM, Pereira WE & Rostad CE (1985) Isolation and identification of four species of quinoline-degrading pseudomonads from a creosote-contaminated site at Pensacola, Florida. Microbios Lett. 29: 147–154.Google Scholar
  3. Blaschke M, Kretzer A, Schäfer C, Nagel M & Andreesen JR (1991) Molybdenum-dependent degradation of quinoline by Pseudomonas putida Chin IK and other aerobic bacteria. Arvh. Microbiol. 155: 164–169.Google Scholar
  4. Bohonos J, Chow TW & Spanpggard RJ (1977) Some observations on biodegradation of pollutants in aquatic systems. Jap. J. Antibiot. 30: S275-S285.Google Scholar
  5. Brockman FJ, Denovan BA, Hicks RJ & Fredrickson JK (1989) Isolation and characterization of quinoline-degrading bacteria from subsurface sediments. Appl. Environ. Microbiol. 55: 1029–1032.Google Scholar
  6. Brown GM & Williamson JM (1982) Biosynthesis of riboflavin, folic acid, thiamin, and pantothenic acid. Adv. Enzymol. 53: 345–381.Google Scholar
  7. Collins MD & Cummins CS (1986). Genus Corynebacterium. In: P. Sneath (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1266–1287). The Williams and Wilkins Co., Baltimore.Google Scholar
  8. Cure GL & Keddie RM (1973) Methods for the morphological examination of aerobic coryneform bacteria. In: Board RG & Lovelock DN (Eds), Sampling — Microbiological Monitoring of Environments (pp. 123–135). Academic Press, New York.Google Scholar
  9. Dobson KR, Stephenson M, Greenfield PF & Bell PRF (1985) Identification and treatability of organics in oil shale retort water. Wat. Res. 19: 849–856.Google Scholar
  10. Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P (Ed), Manual of Methods for General Microbiology (p. 24). American Society for Microbiology, Washington, D.C.Google Scholar
  11. Ensign JC & Rittenberg SC (1963) A crystalline pigment produced from 2-hydroxypyridine by Arthrobacter crystallopoietes n. sp. Archiv. Mikrobiol. 47: 137–153.Google Scholar
  12. Ensign JC & Rittenberg SC (1964) The pathway of nicotinic acid oxidation by a Bacillus sp. J. Biol. Chem. 239: 2285–2291.Google Scholar
  13. Feng Y, Kaiser J-P, Minard RD & Bollag J-M (1994) Microbial transformation of ethylpyridines. Biodegradation. 5: 121–128. Gherna RL, Richardson SH & Rittenberg SC (1965) J. Biol. Chem. 240: 3669–3674.Google Scholar
  14. Godsy EM, Goerlitz DF & Grbic-Galic D (1992) Methanogenic biodegradation of creosote contaminants in natural and simulated ground-water ecosystems. Ground Water. 30: 232–242.Google Scholar
  15. Golovlev EL (1976) Characteristics of the regulation of the microbial transformation of organic compounds. Chem. Abstr. 85: 156250x.Google Scholar
  16. Goodfellow M (1986) Genus Rhodococcus. In: Williams S (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 4 (pp.2362–2371). The Williams and Wilkins Co., Baltimore.Google Scholar
  17. Holmes PE & Rittenberg SC (1972) The bacterial oxidation of nicotine. VII. Partial purification and properties of 2,6-dihydroxypyridine oxidase. J. Biol. Chem. 247: 7622–7627.Google Scholar
  18. Holmes PE, Rittenberg SC & Knackmuss HJ (1972) The bacterial oxidation of nicotine. VIII. Synthesis of 2,3,6-trihydroxypyridine and accumulation and partial characterization of the product of 2,6-dihydroxypyridine oxidation. J. Biol. Chem. 247: 7628–7633.Google Scholar
  19. Houghton C & Cain RB (1972) Formation of pyridinediols (dihydroxypyridines) as intermediates in the degradation of pyridine compounds by micro-organisms. Biochem. J. 130: 879–893.Google Scholar
  20. Jones D & Collins MD (1986) Irregular, nonsporing, Gram-positive rods. In: Sneath P (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1261–1266). The Williams and Wilkins Co., Baltimore.Google Scholar
  21. Jones D & Keddie RM (1986) Genus Brevibacterium. In: Sneath P (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1301–1313). The Williams and Wilkins Co., Baltimore.Google Scholar
  22. Keddie RM (1974) Genus Arthrobacter. In: Buchanan and Gibbons (Eds), Bergy's Manual of Determinative Bacteriology (pp. 618–625). 8 edn. The Williams and Wilkins Co., Baltimore.Google Scholar
  23. Keddie RM, Collins MD & Jones D (1986) Genus Arthrobacter. In: Sneath, P. (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1288–1301). The Williams and Wilkins Co., Baltimore.Google Scholar
  24. Keeny DR & Nelson DW (1982) Nitrogen — Inorganic Forms. In: Page AL (Ed), Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties (pp. 674–676). Soil Science Society of America, MadisonGoogle Scholar
  25. Kloos WE, Tornabene TG & Schleifer KH (1974) Isolation and characterization of micrococci from human skin, including two new species: Micrococus lylae and Micrococcus kristinae. Int. J. Syst. Bacteriol. 24: 79–101.Google Scholar
  26. Kocur M, Bergan T & Mortensen N (1971) DNA base composition of Gram-positive cocci. J. Gen. Microbiol. 69: 69.Google Scholar
  27. Kolenbrander PE, Lotong N & Ensign JC (1976) Growth and pigment production by Arthrobacter pyridinolis n. sp. Arch. Microbiol. 110: 239–245.Google Scholar
  28. Korosteleva LA, Kost AN, Vorob'eva LI, Modyanova LV, Terent'ev PB & Kulikov NS (1981) Microbiological degradation of pyridine and 3-methylpyridine. Appl. Biochem. Microbiol. 17: 276.Google Scholar
  29. Kuhn EP & Suflita JM (1989) Microbial degradation of nitrogen, oxygen and sulfur heterocyclic compounds under anaerobic conditions: Studies with aquifer samples. Environ. Toxicol. Chem. 8: 1149–1158.Google Scholar
  30. Kuhn R, Starr MP, Kuhn DA, Bauer H & Knackmuss HJ (1965) Indigoidine and other bacterial pigments related to 3,3′-bipyridyl. Arch. Mikrobiol. 51: 71–84.Google Scholar
  31. Leenheer JA, Noyes TI & Stuber HA (1982) Determination of polar organic solutes in oil-shale retort water. Environ. Sci. Technol. 16: 714–723.Google Scholar
  32. Leenheer JA & Stuber HA (1981) Migration through soil of organic solutes in an oil-shale process water. Environ. Sci. Technol. 15: 1467–1475.Google Scholar
  33. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.Google Scholar
  34. O'Loughlin EJ, Kehrmeyer SR & Sims GK (1995) Isolation, characterization and substrate utilization of a quinoline-degrading microorganism. Internat. Biodeterior. Biodegradat. 38: 107–118.Google Scholar
  35. Pereira WE and Rostad CE (1985) Investigations of organic contaminants derived from wood-treatment processes in a sand and gravel aquifer near Pensacola, Florida. U.S. Geological Survey Water-Supply Paper 2290. U.S. Geological Survey, Denver, CO.Google Scholar
  36. Pereira WE, Rostad CE, Garbarino JR & Hult MF (1983) Groundwater contamination by organic bases derived from coal-tar wastes. Environ. Toxicol. Chem. 2: 283–294.Google Scholar
  37. Pereira WE, Rostad CE, Leiker TJ, Updegraff DM & Bennett JL. (1988) Microbial hydroxylation of quinoline in contaminated groundwater: Evidence for incorporation of the oxygen atom of water. Appl. Environ. Microbiol. 54: 827–829.Google Scholar
  38. Rogers JE, Riley RG, Li SW, O'Malley ML & Thomas BL (1985) Microbial transformation of alkylpyridines in groundwater. Wat., Air, Soil Pollut. 24: 443–454.Google Scholar
  39. Schwarz G, Senghas E, Erben A, Schäfer E, Lingens F & Höke H (1988) Microbial metabolism of quinoline and related compounds. I. Isolation and characterization of quinoline-degrading bacteria. System. Appl. Microbiol. 10: 185–190.Google Scholar
  40. Shukla OP (1974) Microbial decomposition of α-picoline. Indian J. Biochem. Biophys. 11: 192–200.Google Scholar
  41. Shukla OP (1975) Microbial decomposition of 2-ethylpyridine, 2,4-lutidine and 2,4,6-collidine. Indian. J. Exp. Biol. 13: 574–575.Google Scholar
  42. Shukla OP (1986) Microbial transformation of quinoline by a Pseudomonas sp. Appl. Environ. Microbiol. 51: 1332–1342.Google Scholar
  43. Shukla OP & Kaul SM (1974) A constitutive pyridine degrading system in Corynebacterium sp. Indian J. Biochem. Biophys. 11: 201–207.Google Scholar
  44. Shukla OP & Kaul SM (1975) Succinate semialdehyde, an intermediate in the degradation of pyridine by Brevibacterium sp. Indian J. Biochem. Biophys. 12: 321–330.Google Scholar
  45. Shulka OP (1984) Microbial transformation of pyridine derivatives. J. Sci. Ind. Res. 43: 98–116.Google Scholar
  46. Sims GK & O'Loughlin EJ (1989) Degradation of pyridines in the environment. Crit. Rev. Environ. Control. 19: 309–340.Google Scholar
  47. Sims GK & O'Loughlin EJ (1992) Riboflavin production during growth of Micrococcus luteus on pyridine. Appl. Environ. Microbiol. 58: 3423–3425.Google Scholar
  48. Sims GK & Sommers LE (1985) Degradation of pyridine derivatives in soil. J. Environ. Qual. 14: 580–584.Google Scholar
  49. Sims GK, Sommers LE & Konopka A. (1986). Degradation of pyridine by Micrococcus luteus isolated from soil. Appl. Environ. Microbiol. 51: 963–968.Google Scholar
  50. Smibert RM & Krieg NR (1982) General Characterization. In: Gerhardt P (Ed), Manual of Methods for General Microbiology (pp. 433–434). American Society for Microbiology, Washington, D.C.Google Scholar
  51. Stackebrandt E & Woese CR. (1979) A phylogenic dissection of the family Micrococcaceae. Curr. Microbiol. 2: 317–322.Google Scholar
  52. Stuermer DH, Ng DJ & Morris CJ (1982) Organic contaminants in groundwater near an underground coal gasification site in northeastern Wyoming. Environ. Sci. Technol. 16: 582–587.Google Scholar
  53. Watson GK & Cain RB (1975) Microbial metabolism of the pyridine ring. Metabolic pathways of pyridine degradation by soil bacteria. Biochem. J. 146: 157–172.Google Scholar

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© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Edward J. O'Loughlin
  • Gerald K. Sims
  • Samuel J. Traina

There are no affiliations available

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