Journal of Biomolecular NMR

, Volume 12, Issue 3, pp 417–421 | Cite as

Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes

  • David L. Jakeman
  • Dan J. Mitchell
  • Wendy A. Shuttleworth
  • Jeremy N.S. Evans


Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 13P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 ± 12 Hz and 125 ± 7 Hz respectively, by a variety of methods involving additives and freezing techniques.

EPSP synthase fast freezing narrowing linewidths 13P NMR solid-state NMR trehalose 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • David L. Jakeman
    • 1
  • Dan J. Mitchell
    • 1
  • Wendy A. Shuttleworth
    • 1
  • Jeremy N.S. Evans
    • 1
  1. 1.Department of Biochemistry and BiophysicsWashington State UniversityPullmanU.S.A

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