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Journal of Biomolecular NMR

, Volume 11, Issue 2, pp 205–212 | Cite as

Solvent Exchange Rates of Side-chain Amide Protons in Proteins

  • Ponni Rajagopal
  • Bryan E. Jones
  • Rachel E. Klevit
Article

Abstract

Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.

HPr side-chain amide proton solvent exchange rates temperature coefficients 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Ponni Rajagopal
    • 1
  • Bryan E. Jones
    • 1
  • Rachel E. Klevit
    • 1
  1. 1.Department of BiochemistryUniversity of WashingtonSeattleU.S.A

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