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Journal of Biomolecular NMR

, Volume 11, Issue 2, pp 199–203 | Cite as

Measurement of Three-bond, 13C′-13J Couplings in Human Ubiquitin by a Triple Resonance, E. COSY-type NMR Technique

  • Jin-Shan Hu
  • Ad Bax
Article

Abstract

A [CO]HN(CA)CB-E.COSY pulse scheme is described for measurement of three-bond couplings, 3JC′Cβ, between carbonyl and aliphatic Cβ carbons in ubiquitin, uniformly enriched with 13C and 15N. A Karplus relation, 3JC′Cβ = 1.28 cos2(φ - 120°) -1.02 cos(φ - 120°) +0.30 Hz, is obtained by correlating the 3JC′Cβ values measured for human ubiquitin with backbone φ angles from its crystal structure. As predicted, the new Karplus parametrization yields 3JC′Cβ values slightly larger than previously obtained by quantitative J correlation [Hu, J.-S. and Bax, A. (1997) J. Am. Chem. Soc., 119, 6360-6368], but considerably smaller than what has been reported on the basis of other E.COSY-type measurements carried out on flavodoxin.

carbon-carbon J coupling E. COSY Karplus equation φ angle 3D NMR ubiquitin 

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Supplementary material

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Jin-Shan Hu
    • 1
  • Ad Bax
    • 1
  1. 1.Laboratory of Chemical PhysicsNational Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of HealthBethesdaU.S.A

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