Virus Genes

, Volume 21, Issue 3, pp 227–232

Genetic Diversity of Apodemus agrarius-Borne Hantaan Virus in Korea

  • Jin-Won Song
  • Luck Ju Baek
  • Sang Hyun Kim
  • Eun Young Kho
  • Jong Hun Kim
  • Richard Yanagihara
  • Ki-Joon Song


Hantaan (HTN) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (HFRS), was first isolated in 1976 from lung tissues of striped field mice (Apodemus agrarius) captured in Songnae-ri, Kyungki-do, Korea. To clarify the genetic diversity and phylogenetic relationship among Korean strains of HTN virus, viral sequences of the partial S and M segments were amplified from lung tissues of 24 seropositive striped field mice captured between 1989 and 1998 at 11 sites in South Korea. The 771-nucleotide (nt) S segment sequences (coordinates 432 to 1202) of HTN virus strains from Yangju-kun differed by 10 to 40 nt (1.3 to 5.2%) from virus strains from Pocheon-kun, Songnae-ri and Nonsan-kun. Similar degrees of genetic variation were found in the G1 and G2 glycoprotein-encoding M segment. Phylogenetic trees, based on the partial S and M segments and generated by the maximum parsimony and neighbor-joining methods, demonstrated that virus strains from various geographic regions in South Korea showed a tendency to form two phylogenetic subgroups and were evolutionarily distinct from HTN virus strains from the People’s Republic of China.

genetic diversity Hantaan virus Hantavirus RT-PCR 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lee H.W., Lee P.W., and Johnson K.M., J Infect Dis 137, 298–308, 1978.Google Scholar
  2. 2.
    Nichol S.T., Spiropoulou C.F., Morzunov S., Rollin P.E., Ksiazek T.G., Feldmann H., Sanchez A., Zaki S., Childs J., and Peters C.J., Science 262, 914–917, 1993.Google Scholar
  3. 3.
    Schmaljohn C.S., Jennings G.B., Hay J., and Dalrymple J.M., Virology 155, 633–643, 1986.Google Scholar
  4. 4.
    Schmaljohn C.S., Schmaljohn A.L., and Dalrymple J.M., Virology 157, 31–39, 1987.Google Scholar
  5. 5.
    Schmaljohn C.S., Nucleic Acids Res 18, 6728, 1990.Google Scholar
  6. 6.
    Lee H.W., Calisher C., and Schmaljohn C.S. (ed). Manual of hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. WHO Collaborating Center for Virus Reference and Research (Hantaviruses). Aian Institute for Life Sciences, Seoul, Korea, 1998, p. 11Google Scholar
  7. 7.
    Xiao S.Y., Yanagihara R., Godec M.S., Eldadah Z.A., Johnson B.K., Gajdusek D.C., and Asher D.M., J Med Virol 33, 277–282, 1991.Google Scholar
  8. 8.
    Xiao S.Y., Chu Y.K., Knauert F.K., Lofts R., Dalrymple J.M., and LeDuc J.W., J Gen Virol 73, 567–573, 1992.Google Scholar
  9. 9.
    Swofford D.L., PAUP: phylogenetic analysis using parsimony, version 4.0b. Sinauer Associates, Inc., Sunderland, MA. 1998.Google Scholar
  10. 10.
    Plyusnin A., Vapalahti O., Lehvaslaiho H., Apekina N., Mikhailova T., Gavrilovskaya I., Laakkonen J., Niemimaa J., Henttonen H., Brummer-Korvenkontio M., and Vaheri A., Virus Res 38, 25–41, 1995.Google Scholar
  11. 11.
    Plyusnin A., Vapalahti O., Ulfves K., Lehvaslaiho H., Apekina N., Gavrilovskaya I., Blinov V., and Vaheri A., J Gen Virol 75, 405–409, 1994.Google Scholar
  12. 12.
    Xiao S.Y., LeDuc J.W., Chu Y.K., and Schmaljohn C.S., Virology 198, 205–217, 1994.Google Scholar
  13. 13.
    Liang M., Li D., Xiao S.Y., Hang C., Rossi C.A., and Schmaljohn C.S., Virus Res 31, 219–233, 1994.Google Scholar
  14. 14.
    Xiao S.Y., Liang M., and Schmaljohn C.S., J Gen Virol 74, 1657–1659, 1993.Google Scholar
  15. 15.
    Gligic A., Dimkovic N., Xiao S.Y., Buckle G.J., Jovanovic D., Velimirovic D., Stojanovic R., Obradovic M., Diglisic G., Micic J., Asher D.M., LeDuc J.W., Yanagihara R., and Gajdusek D.C., J Infect Dis 166, 113–120, 1992.Google Scholar
  16. 16.
    Avsic-Zupanc T., Xiao S.Y., Stojanovic R., Gligic A., van der Groen G., and LeDuc J.W., J Med Virol 38, 132–137, 1992.Google Scholar
  17. 17.
    Lundkvist A., Wiger D., Horling J., Sjolander K.B., Mehl R., Vaheri A., and Plyusnin A., J Gen Virol 79, 2603–2614, 1998.Google Scholar
  18. 18.
    Plyusnin A., Vapalahti O., Lankinen H., Lehväslaiho H., Apekina N., Myasnikov Y., Kallio-Kokko H., Henttonen H., Lundkvist A., Brummer-Korvenkontio M., Gavrilovskaya I., and Vaheri A., J Virol 68, 7833–7839, 1994.Google Scholar
  19. 19.
    Sibold C., Sparr S., Schulz A., Labuda M., Kozuch O., Lysy J., Kruger D.H., and Meisel H., Virus Genes 10, 277–281, 1995.Google Scholar
  20. 20.
    Plyusnin A., Cheng Y., Vapalahti O., Pejcoch M., Unar J., Jelinkova Z., Lehvaslaiho H., Lundkvist A., and Vaheri A., Virus Res 39, 237–250, 1995.Google Scholar
  21. 21.
    Vapalahti O., Lundkvist A., Kukkonen S.K., Cheng Y., Gilljam M., Kanerva M., Manni T., Pejcoch M., Niemimaa J., Kaikusalo A., Henttonen H., Vaheri A., and Plyusnin A., J Gen Virol 77, 3063–3067, 1996.Google Scholar
  22. 22.
    Song K.-J., Kim S.Y., Baek L.J., and Lee Y.J., J Korean Soc Microbiol 29, 675–683, 1994.Google Scholar
  23. 23.
    Kariwa H., Isegawa Y., Arikawa J., Takashima I., Ueda S., Yamanishi K., and Hashimoto N., Virus Res 33, 27–38, 1994.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Jin-Won Song
    • 1
  • Luck Ju Baek
    • 1
  • Sang Hyun Kim
    • 1
  • Eun Young Kho
    • 1
  • Jong Hun Kim
    • 1
  • Richard Yanagihara
    • Ki-Joon Song
      • 1
    1. 1.Department of Microbiology, College of Medicine and Institute for Viral DiseasesKorea UniversitySeoul 136–705Korea

    Personalised recommendations