Virus Genes

, Volume 19, Issue 1, pp 85–88 | Cite as

Sequence Analysis of the Gene Encoding VP4 of a Bovine Group C Rotavirus: Molecular Evidence for a New P Genotype

  • B. Jiang
  • J.R. Gentsch
  • H. Tsunemitsu
  • L.J. Saif
  • R.I. Glass


Nucleotide sequence of the bovine group C rotavirus Shintoku strain gene 3 was determined. Segment 3 is 2253 nucleotides (nt) in length and contains a long open reading frame (ORF) beginning at nt 22 and terminating at nt 2223. This ORF encodes a polypeptide of 733 amino acids with a predicted molecular mass of 83 kDa. The deduced gene 3 amino acid sequence shares 79% and 73% identities with VP4 of the porcine Cowden and human Bristol strains, respectively. Lack of high amino acid sequence homology in VP4 of bovine, porcine, and human group C rotaviruses indicates that the Shintoku strain represents a new P genotype.

rotavirus Shintoku Cowden Bristol VP4 P genotpye 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Clem R.J., Fechheimer M., and Miller L.K., Science 254, 1388±1390, 1991.Google Scholar
  2. 2.
    Clem R.J. and Miller L.K., J Virol 67, 3730±3738, 1993.Google Scholar
  3. 3.
    Clem R.J., and Miller L.K., Mol Cell Biol 14, 5212±5222, 1994.Google Scholar
  4. 4.
    Crook N.E. Clem R.J., and Miller L.K., J Virol 67, 2168±2174, 1993.Google Scholar
  5. 5.
    Birnbaum M.J., Claus R.J., and Miller L.K., J Virol 68, 2521±2528, 1994.Google Scholar
  6. 6.
    Friesen P.D. and Miller L.K., J Virol 61, 2264±2272, 1987.Google Scholar
  7. 7.
    Ayres M.D., oward S.C., Kuzio J., Lopez-Ferber M., and Possee R.D., Virology 202, 586±605, 1994.Google Scholar
  8. 8.
    Kamita S.G., Majima K., and Maeda S., J Virol 67, 455±463, 1993.Google Scholar
  9. 9.
    Clem R.J., Hardwick J.M., and Miller L.K., Cell Death Differ 3, 9±16, 1996.Google Scholar
  10. 10.
    Miller L.K., J Cell Physiol 173(2), 178±182.Google Scholar
  11. 11.
    Hershberger P.A., Dickson J.A., and Friesen P.D., J Virol 66, 5525±5533, 1992.Google Scholar
  12. 12.
    Lu A. and Miller L.K., J Virol 69, 975±982, 1995.Google Scholar
  13. 13.
    Ahrens C.H., Russell R.L.Q, Funk C.J., Evans T.J., Harwood S.H., and Rohrmann G.F., Virology 229, 381±399, 1997.Google Scholar
  14. 14.
    Duckett C.S., Nava V.E., Gedrich R.W., Clem R.J., Van Dongen J.L., Gil®llan M.C., Shiels H., Hardwick J.M., and Thompson C.B., EMBO J 15, 2685±2694, 1996.Google Scholar
  15. 15.
    Hawkins C.J., Uren A.G., Hacker G., Medcalf R.L., and Vaux D.L., Proc Natl Acad Sci USA 93, 13786±13790, 1996.Google Scholar
  16. 16.
    Uren A.G., Pakusch M., Hawkins C.J., Puls K.L., and Vaux D.L., Proc Natl Acad Sci USA 93, 4974±4978, 1996.Google Scholar
  17. 17.
    Manji G.A., Hozak R.R., LaCount D.J., and Friesen P.D., J Virol 71, 4509±4516, 1997.Google Scholar
  18. 18.
    Summers M.D. and Paschke J.D., J Invert Pathol 16, 227–240, 1970.Google Scholar
  19. 19.
    Bideshi D.K., Hice R.H., Ge G., and Federici B.A., J Gen Virol 79, 1309–1319, 1998.Google Scholar
  20. 20.
    Sanger F., Nicklen S., and Coulson A.R., Proc Natl Acad Sci USA 74, 5463–5467, 1977.Google Scholar
  21. 21.
    Hashimoto Y., Hayashi K., Okuno Y., Hayakawa T., Saimoto A., Granados R.R., and Matsumoto T., J Gen Virol 77, 555–563, 1996.Google Scholar
  22. 22.
    O'Reilly D.R., Miller L.K., and Luckow V.A., Baculovirus expression vectors. A Laboratory Manual. Oxford University Press, New York, 1994, pp. 132–134.Google Scholar
  23. 23.
    Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403–410, 1990.Google Scholar
  24. 24.
    Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res 1, 387–395, 1984.Google Scholar
  25. 25.
    Thompson J.D., Higgins D.G., and Gibson T.J., Nuc Acids Res 22, 4673–4680, 1994.Google Scholar
  26. 26.
    Felsenstein J., PHYLIP version 3.57c. Department of Genetics, Washington, Seattle, 1995.Google Scholar
  27. 27.
    Federici B., in Beckage N., Thompson S.N., and Federici B.A. (eds). Parasites and Pathogens of Insects, vol. 2. Academic Press, San Diego, 1993, pp. 81–101.Google Scholar
  28. 28.
    Freemont P.S., Hanson I.M., and Trowsdale J., Cell 64, 483–484, 1991.Google Scholar
  29. 29.
    Hu Z.H., Arif B.M, Sun J.S., Chen X.W., Zuidema D., Goldbach R.W., and Vlak, J.M., Virus Res 55, 71–82, 1988.Google Scholar
  30. 30.
    Tanada Y., J Insect Pathol 6, 378–380.Google Scholar
  31. 31.
    Tanada Y. and Leutenegger R., J Invertebr Pathol 10, 39–47, 1968.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • B. Jiang
    • 1
  • J.R. Gentsch
    • 1
  • H. Tsunemitsu
    • 2
  • L.J. Saif
    • 3
  • R.I. Glass
    • 1
  1. 1.Viral Gastroenteritis Section, Division of Viral and Rickettsial DiseasesCenters for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human ServicesAtlantaU.S.A
  2. 2.Shichinohe Research UnitNational Institute of Animal HealthAomoriJapan
  3. 3.Food Animal Health Research Program, Ohio Agricultural Research and Development CenterOhio State UniversityWoosterU.S.A

Personalised recommendations