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Virus Genes

, Volume 20, Issue 3, pp 237–241 | Cite as

Sequence Analysis of Pns11, a Nonstructural Protein of Rice Gall Dwarf Virus, and its Expression and Detection in Infected Rice Plants and Vector Insects

  • Yusuke Moriyasu
  • Koichi Ishikawa
  • Akira Kikuchi
  • Shigeo Imanishi
  • Shuichiro Tomita
  • Katsumi Akutsu
  • Toshihiro Omura
Article

Abstract

The nucleotide sequence of genome segment S11 of rice gall dwarf virus (RGDV), a member of Phytoreovirus, was determined. The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses, which are other members of Phytoreovirus. A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA. An antiserum raised against this protein reacted with a protein of approximately 40 kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects. However, the antiserum did not react with purified viral proteins. These results suggest that S11 encodes a nonstructural protein of RGDV. This protein was named Pns11.

RGDV/Phytoreovirus/Pns11 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Yusuke Moriyasu
    • 1
  • Koichi Ishikawa
    • 1
  • Akira Kikuchi
    • 1
  • Shigeo Imanishi
    • 2
  • Shuichiro Tomita
    • 2
  • Katsumi Akutsu
    • 3
  • Toshihiro Omura
    • 1
  1. 1.National Agriculture Research CenterIbarakiJapan
  2. 2.National Institute of Sericultural and Entomological ScienceIbarakiJapan
  3. 3.Faculty of AgricultureIbaraki UniversityIbarakiJapan

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