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Journal of Applied Phycology

, Volume 12, Issue 3–5, pp 435–439 | Cite as

Antioxidant activities of phycocyanobilin prepared from Spirulina platensis

  • Takashi Hirata
  • Mikiya Tanaka
  • Masaki Ooike
  • Teppei Tsunomura
  • Morihiko Sakaguchi
Article

Abstract

The antioxidative activity of phycocyanobilin fromSpirulina platensis was evaluated againstoxidation of methyl linoleate in a hydrophobic systemor with phosphatidylcholine liposomes. Phycocyanobilin as well as phytochemicals includingα-tocopherol, caffeic acid and zeaxanthin,effectively inhibited the peroxidation of methyllinoleate and produced a prolonged induction period.Oxidation of phosphatidylcholine liposomes was alsocontrolled markedly by adding phycocyanobilin orα-tocopherol. Phycocyanobilin was distributedoutside in the liposomes to scavenge radicals fromAAPH and to prevent initiation of radical chainreactions. When the concentrations of phycocyanin andphycocyanobilin in the reaction mixture were adjustedequally on a phycocyanobilin basis, the activity ofphycocyanobilin was almost the same as that ofphycocyanin in the AAPH-containing reaction mixture.The antioxidizing action of phycocyanin prepared fromspray-dried Spirulina almost agreed with thatfrom fresh Spirulina in the AAPH-containingreaction mixture. These results suggest thatphycocyanobilin is responsible for the majority of theantioxidative activity of phycocyanin and may act asan effective antioxidant in a living human body.

active oxygen blue-green alga cyanobacteria liposome oxidation phycobilin phycobiliprotein phycocyanin radical 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Takashi Hirata
    • 1
  • Mikiya Tanaka
    • 1
  • Masaki Ooike
    • 1
  • Teppei Tsunomura
    • 1
  • Morihiko Sakaguchi
    • 1
  1. 1.Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversityKyotoJapan

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