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Journal of Applied Phycology

, Volume 12, Issue 3–5, pp 291–300 | Cite as

Hydrogen production by microalgae

  • John R. Benemann
Article

Abstract

The production of H2 gas from water and sunlightusing microalgae, `biophotolysis', has been a subjectof applied research since the early 1970s. A numberof approaches have been investigated, but most provedto have fundamental limitations or requireunpredictable research breakthroughs. Examples areprocesses based on nitrogen-fixing microalgae andthose producing H2 and O2 simultaneously fromwater (`direct biophotolysis'). The most plausibleprocesses for future applied R & D are those whichcouple separate stages of microalgal photosynthesisand fermentations (`indirect biophotolysis'). Theseinvolve fixation of CO2 into storagecarbohydrates followed by their conversion to H2by the reversible hydrogenase, both in dark andpossibly light-driven anaerobic metabolic processes. Based on a preliminary engineering and economicanalysis, biophotolysis processes must achieve closeto an overall 10% solar energy conversion efficiencyto be competitive with alternatives sources ofrenewable H2, such as photovoltaic-electrolysisprocesses. Such high solar conversion efficiencies inphotosynthetic CO2 fixation could be reached bygenetically reducing the number of light harvesting(antenna) chlorophylls and other pigments inmicroalgae. Similarly, greatly increased yields ofH2 from dark fermentation by microalgae could beobtained through application of the techniques ofmetabolic engineering. Another challenge is toscale-up biohydrogen processes with economicallyviable bioreactors.Solar energy driven microalgae processes forbiohydrogen production are potentially large-scale,but also involve long-term and economically high-riskR&D. In the nearer-term, it may be possible tocombine microalgal H2 production with wastewatertreatment.

biophotolysis fermentations hydrogen microalgae photobioreactors photosynthetic efficiencies 

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References

  1. Aoyama K, Uemura I, Miyake J, Asada Y (1997) Fermentative metabolism to produce hydrogen gas and organic compoundsin a cyanobacterium, Spirulina platensis. J. Ferment. Bioengng 83: 17-20.Google Scholar
  2. Arnon DI, Mitsui A, Paneque A (1961) Photoreduction of hydrogen gas coupled with photosynthetic phosphorylation. Science 134: 1425.Google Scholar
  3. Benemann JR (1973) A Model System for nitrogen fixation and hydrogen evolution by non-heterocystous blue-green algae. Fed. Proc. 32: 632.Google Scholar
  4. Benemann JR (1977) Hydrogen and methane production through microbial photosynthesis. In Buvet R (ed.), Living Systems as Energy Converters. Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 285-298.Google Scholar
  5. Benemann JR (1990) The future of microalgae biotechnology. In Cresswell RC, Rees TAV, Shah N (eds), Algal Biotechnology, Longman, London: pp. 317-337.Google Scholar
  6. Benemann JR (1993) Utilization of carbon dioxide from fossil fuel-burning power plants with biological systems. Energy Conserv. Mgmt. 34: 999-1004.Google Scholar
  7. Benemann JR (1994) Feasibility analysis of photobiological hydrogen production. In Block DL, Versiroglu TN (eds.), Hydrogen Energy Progress X, Proc. 10th World Hydrogen Energy Conf., Cocoa Beach, Florida, pp. 931-940.Google Scholar
  8. Benemann JR (1996) Hydrogen biotechnology: Progress and prospects. Nature Biotech. 14: 1101-1103.Google Scholar
  9. Benemann JR (1998a) The technology of biohydrogen. In Zaborksy O (ed.) Biohydrogen, Plenum Press, pp. 19-30.Google Scholar
  10. Benemann JR (1998b) Processes Analysis and Economics of Biophotolysis of Water. A Preliminary Assessment. Report to the International Energy Agency Hydrogen Program, Annex 10, Photoproduction of Hydrogen IEA/H2/10/TR-2-98.Google Scholar
  11. Benemann JR, Berenson JA, Kaplan NO, Kamen MD (1973) Hydrogen evolution by a chloroplast-ferredoxin-hydrogenase system. Proc. Nat. Acad. Sci. USA 70: 2317-2320.Google Scholar
  12. Benemann JR, Weare NM (1974) Hydrogen evolution by nitrogen-fixing Anabaena cylindrica cultures. Science 184: 1917-1918.Google Scholar
  13. Benemann JR, Yoch DC, Valentine RC, Arnon DI (1969) The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier. Proc. Nat. Acad. Sci. USA 64: 1079-1086.Google Scholar
  14. Berenson JA, Benemann JR (1977) Immobilization of hydrogenase and ferrodoxins on glass beads. FEBS Letters 76: 105-107.Google Scholar
  15. Bishop NI (1966) Partial reactions of photosynthetsis and photoreduction. Ann. Rev. Plant Physiol., 17: 185-208.Google Scholar
  16. Block DL, Melody I (1992) Efficiency and cost goals for photoenhanced hydrogen production processes. Int. J. Hydrogen Energy 17: 853-861.Google Scholar
  17. Boichenko VA, Hoffman P (1994) Photosynthetic hydrogen production in Prokaryotes and Eukaryotes: Occurence, mechanism and functions. Photosynthetica 30: 527-552.Google Scholar
  18. Bolton JR (1996) Solar Photoproduction of Hydrogen. Report to the International Energy Agency, under Agreement on the Production and Utilization of Hydrogen IEA/H2/TR-96 September 1996.Google Scholar
  19. Burlew D (1953) Algae Culture from Laboratory to Pilot Plant. Carnegie Institute of Washington, Washington D.C.Google Scholar
  20. Gaffron H, Rubin J (1942) Fermentative and photochemical production of hydrogen in algae. J. gen. Physiol. 26: 219-240.Google Scholar
  21. Ghirardi ML, Togasaki RK, Seibert M (1997) Oxygen sensitivity of algal hydrogen production. Appl. Biochem. Biotech. 63-65: 141-151.Google Scholar
  22. Gibbs M, Hollaender A, Kok B, Krampitz LO, San Pietro A (1973) Proceedings of the Workshop on Bio Solar Hydrogen Conversion. September 5-6 1973, Bethesda Maryland.Google Scholar
  23. Greenbaum, E (1988) Energetic efficiency of hydrogen photoevolution by algal water splitting. Biophys. J. 54: 365-368.Google Scholar
  24. Hallenbeck PC, Kochian LV, Weissman JC, Benemann JR (1978) Solar energy conversion with hydrogen producing cultures of the blue-green alga, Anabaen cylindrica. Biotech. Bioengng Symp. No. 8, 283-297.Google Scholar
  25. Hallenbeck, PC, Benemann JR (1979) Hydrogen from algae. In Barber J (ed.) Photosynthesis in Relation to Model Systems. Elsevier/North-Holland Biomedical-Press, pp. 333-364.Google Scholar
  26. Happe RP, Roseboom W, Plerik AJ, Albracht SPL, Bagley KA (1997) Biological activation of hydrogen. Nature 385: 126.Google Scholar
  27. Ikuta Y, Akano T, Shioji N, Maeda I (1998) Hydrogen Production by</del> Photosynthetic Microogranisms. In O. Zaborsky, ed., Biohydrogen, Plenum Press, New York, pp. 319-328.Google Scholar
  28. Jackson DD, Ellms JW (1896) On odors and tastes of surface waters with special reference to Anabaena, a microscopial organsim found in certain water supplies of Massachusetts, Rep. Mass. State Board Health 1896: 410-420.Google Scholar
  29. Keasling JD, Benemann JR, Pramanik J, Carrier TA, Jones KL, VanDien SJ (1998) A toolkit for metabolic engineering of bacteria: application to hydrogen production. In Zaborsky O (ed.), BioHydrogen, Plenum Press, pp. 87-98.Google Scholar
  30. Kessler E (1974) Hydrogenase, photoreduction, and anaerobic growth. In Stewart WDP (ed.), Algal Physiology and Biochemistry. Blackwell, Oxford, pp. 456-473.Google Scholar
  31. Kok B (1973) Photosynthesis. I Gibbs M, (ed.), Proceeding of the Workshop on Bio Solar Hydrogen Conversion. September 5-6 1973, Bethesda Maryland, pp. 22-30.Google Scholar
  32. Lambert GR, Smith GD (1981) The Hydrogen metabolism of cyanobacteria (blue-green algae). Biol. Rev. 56: 589-660.Google Scholar
  33. Markov SA, Bazin M, Hall DO (1995) The potential of using cyanobacteria in photobioreactors for hydrogen production. Adv. Biochem. Eng. 52: 59-86.Google Scholar
  34. McBride AC, Lien S, Togasaki RK, San Pietro A (1977) Mutational analysis of Chlamydomonas reinhardi: Application to biological eneragy conversion. In Mitsui A, Miyachi S, San Pietro A, Tamura S (eds), Biological Solar Energy Conversion, Academic Press, New York, pp. 77-86.Google Scholar
  35. McTavish H, Sayavedra-Soto LA, Arp DJ (1995) Substitution of Azotobacter vinelandii hydrogenase small subunit cysteins by serines can create insensitivity to inhibition by O2 and preferentially damages H2 oxidation over H2 evolution. J. Bact. 177: 3960-3964.Google Scholar
  36. Melis A (1991) Dynamics of photosynthetic membrane composition and function. Biochim. Biophys. Acta (Reviews on Bioenergetics) 1058: 87-106.Google Scholar
  37. Melis A, Neidhardt J, Baroli I, Benemann JR (1998) Maximizing photosynthetic productivity and light utilization in microalgae by minimizing the light-harvesting chlorophyll antenna size of the photosystems. In Zaborsky O. (ed.) Biohydrogen Plenum Press, New York, pp. 41-52.Google Scholar
  38. Melis A, Neidhardt J, Benemann JR (1999) Dunaliella salina (Chlorophyta) with small chlorophyll antenna sizes exhibit higher photosynthetic productivities and photon use effeiciencies than normally pigmented cells. J. App. Phycol. 10: 515-525.Google Scholar
  39. Melis A, Zhang, L, Forestier M, Ghirardi ML, Seiber M (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolutin in the green alga Chlamydomonas reinhardtii. J. Plant Physiol. 122: 127-135.Google Scholar
  40. Mitsui A (1992) Biological hydrogen photoproduction. In Proc. 1992 DOE/NREL Hydrogen Program Review, May 6-7 1992, Honolulu Hawaii, NREL/CP-450-4972, pp. 129-156.Google Scholar
  41. Miyamoto K (1994) Hydrogen production by photosynthetic bacteria and microalgae. In Murooka Y, Imanaka T (eds.), Recombinant Microbes for Industrial and Agricultural Applications. Marcel Dekker, New York, pp. 771-786.Google Scholar
  42. Myers J (1957) Algal culture. Encyclopedia of Chemical Technology. Interscience, NY, pp. 649-680.Google Scholar
  43. Nakajima Y, Ueda R (1997) Improvement of photosynthesis in dense microalgal suspension by reduction of light harvesting pigment. J. appl. Phycol. 9: 503-510.Google Scholar
  44. Nakajima Y, Ueda R (1999) Improvement of microalgal photosynthetic productivity by reducing the content of light harvesting pigment. J. appl. Phycol. 11: 195-201.Google Scholar
  45. Nakajima Y, Ueda R (2000) The effect of reducing light-harvesting pigment on marine microalgal productivity. J. appl. Phycol. 12: 285-290.Google Scholar
  46. Nandi R, Segupta S (1998) Microbial production of hydrogen: An overview. Critical Re. Microbiol. 24: 61-84.Google Scholar
  47. Neidhardt J, Benemann JR, Baroli I, Melis A (1998) Maximizing photosynthetic productivity and light utilization in microalgae by minimizing the light-harvesting chlorophyll antenna size of the photosystems. Photosynthesis Res. 56: 175-184.Google Scholar
  48. Oswald WJ, Goluek CG (1960) Biological Energy Conversion System. Adv. appl. Microbiol. 2: 223-232.Google Scholar
  49. Pauss A, Andre G, Perrier M, Guiot SR, (1990) Liquid-to-gas mass transfer in anaerobic processes: Inevitable transfer limitations of methane and hydrogen in the biomethanation process. Appl. environ. Microbiol. 56: 1636-1644.Google Scholar
  50. Peters JW, Lanzilotta WN, Lemon BJ, Seefeldt LC (1998) X-ray crystal structure of the Fe-only hydrogenase (Cpl) from Clostridium pasteurianum to 1.8 angstrom resolution. Science 282: 1853-1858.Google Scholar
  51. Polle J, Kanakagiri S, Benemann JR, Melis A (2000), Maximizing photosynthetic efficiencies and hydrogen production in microalga cultures. In Miyake J, San Pietro A (eds), Biohydrogen 99.Google Scholar
  52. Radway J, Yoza BA, Benemann JR, Chini-Zitelli G, Malda J, Babcock RW Jr., Tredici M, Zaborsky O (1999) Evaluation of a near-horizontal tubular photobioreactorsystem in Hawaii. (Abstract) In Algae and Human Affaris in the 21st Century 8th Int. Conf. on Applied Algology, 26 September to 1 October 1999, Motecatini Terme, Italy.Google Scholar
  53. Sasikala K, Ramana ChV, Rao PR, Kovacs KL (1993) Anoxygenic phototrophic bacteria: physiology and advances in hydrogen technology. Adv. appl. Microbiol. 38: 211-295Google Scholar
  54. Schulz R (1996) Hydrogenases and hdrogen production in eucaryotic organisms and cyanobacteria. J. mar. Biotech. 4: 15-22.Google Scholar
  55. Spruit CJP (1958) Simultaneous photoproduction of hydrogen and oxygen byChlorella. Mededel. Landbouwhogeschool Wageningen 58: 1-17.Google Scholar
  56. Thauer R (1976) Limitation of microbial hydrogen formation via fermentation. In Schlegel HG and Barnea J (eds), Microbial Energy Conversion. Erich Goltze, Gottingen, Germany, pp. 201-294.Google Scholar
  57. Tredici MR, Zittelli GC, Benemann JR (1998) A tubular internal gas exchange hydrogen production: Preliminary cost analysis. In Zaborsky O (ed.), BioHydrogen. Plenum Press, New York, pp. 391-402.Google Scholar
  58. Turpin DH, Layzell DB, Elrifi IR (1985) Modeling the carbon economy of Anabaena flos-aquae. Plant Physiol. 78: 74-752.Google Scholar
  59. Ueno Y, Morimoto M, Ootsuka S, Kawai T, Satou S (1995) Process for the Production of Hydrogen by Microorganisms and for Wastewater Treatment. U.S. Patent, 5, 464, 539 (November, 7, 1995).Google Scholar
  60. Weare NM, Benemann JR (1973) Nitrogen fixation by Anabaena cylindrica. I. Localization of nitrogen fixation in heterocysts. Arch. Microbiol. 90: 323-332.Google Scholar
  61. Weare NM, Benemann JR (1974). Nitrogenase activity and photosynthesis by Plectonema boryanum 594. J. Bacteriol. 119: 258-268.Google Scholar
  62. Weaver PF, Lien S, Seibert M (1980) Photobiological production of hydrogen. Solar Energy 24: 3-45.Google Scholar
  63. Weissman JC, Benemann JR (1977) Hydrogen production by nitrogen-fixing cultures of Anabaena cylindrica. Appl. environ. Microbiol. 33: 123-131.Google Scholar
  64. Zaborsky O (ed.) (1988) Biohydrogen. Plenum Press New York.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • John R. Benemann
    • 1
  1. 1.Department of Plant and Microbial BiologyUniversity of California BerkeleyBerkeleyUSA

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