Journal of Applied Phycology

, Volume 12, Issue 3–5, pp 395–400 | Cite as

Spirulina cultivation in digested sago starch factory wastewater

  • S.M. Phang
  • M.S. Miah
  • B.G. Yeoh
  • M.A. Hashim


Wastewater arising from the production of sago starchhas a high carbon to nitrogen ratio, which is improvedwith anaerobic fermentation in an upflow packed beddigester. The digested effluent with an average C: N:P ratio of 24: 0.14: 1 supported growth of Spirulina platensis (Arthrospira) with anaverage specific growth rate (μ) of 0.51day-1 compared with the average μ of 0.54day-1 in the inorganic Kosaric Medium in a highrate algal pond. Supplementation with 6 mM urea and2.1 mM K2HPO4 produced gross biomassproductivity of 14.4 g m-2 day-1. Aflow-rate of 24 cm s-1 increased the μ andgross biomass productivity (18 g m-2 day-1). The highest crude protein, carbohydrate and lipidcontents of the biomass were 68%, 23% and 11%,respectively. Percentage reductions in chemicaloxygen demand, ammoniacal-nitrogen and phosphatelevels of the digested effluent reached 98.0%, 99.9%and 99.4% respectively. The HRAP offers a goodtreatment system for sago starch factory wastewater.

Spirulina sago starch factorywastewater treatment high rate algal pond biomass 


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  1. APHA (1989) Standard Methods for the Examination of Water and Wastewater. 17th edition. American Public Health Association, Washington, DC.Google Scholar
  2. Chew TY, Shim YL (1993) Sago processing wastes. In Yeoh BG, Phang SM, Zaid Isa, Azni Idris, Maktab Mohamed (eds), Waste Management in Malaysia. Current Status and Prospects for Bioremediation. Ministry of Science, Technology and the Environment, pp 159-167.Google Scholar
  3. Chu WL, Phang SM, Goh SH (1995) Influence of carbon source on growth, biochemical composition and pigmentation of Ankistrodesmus convolutus. J. appl. Phycol. 7: 59-64.Google Scholar
  4. Chu WL, Phang SM, Goh SH (1996) Environmental effects on growth and biochemical composition of Nitzschia inconspicua Grunow. J. appl. Phycol. 8: 389-396.Google Scholar
  5. Laliberte G, Olguin EJ, de la Noue J (1997) Mass cultivation and wastewater treatment using Spirulina. In Vonshak A (ed.), Spirulina platensis (Arthrospira) Physiology, Cell Biology and Biotechnology. Taylor and Francis, London, pp. 159-174.Google Scholar
  6. Phang SM, Chu WL (1999) University of Malaya Algae Culture Collection. Catalogue of Strains. Institute of Postgraduate Studies and Research Bibliographies and Research Guides: BPP. Bil.2, University of Malaya, Kuala Lumpur, 77 pp.Google Scholar
  7. Phang SM, Ong KC (1988) Algal biomass production in digested palm oil mill effluent. Biol. Wastes 25: 177-191.Google Scholar
  8. Tanticharoen M, Bunnag B, Vonshak A (1993) Cultivation of Spirulina using secondary treated starch wastewater. Australasian Biotechnol. 3: 223.Google Scholar
  9. Torzillo G, Sacchi A, Materassi R (1991) Temperature as an important factor affecting productivity and night biomass loss in Spirulina platensis grown outdoors in tubular photobioreactors. Bioresource Technol. 38: 95-100.Google Scholar
  10. Vonshak A (1997) Spirulina platensis (Arthrospira) Physiology, Cell Biology and Biotechnology. Taylor and Francis, London, 233 pp.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • S.M. Phang
    • 1
  • M.S. Miah
    • 2
  • B.G. Yeoh
    • 3
  • M.A. Hashim
    • 4
  1. 1.Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia
  2. 2.Institute of Postgraduate Studies and ResearchUniversity of MalayaKuala LumpurMalaysia
  3. 3.SIRIMShah Alam, SelangorMalaysia
  4. 4.Department of Chemical EngineeringUniversity of MalayaKuala LumpurMalaysia

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