Plant Foods for Human Nutrition

, Volume 55, Issue 4, pp 347–355 | Cite as

Anticarcinogenic effects of curry leaves in dimethylhydrazine-treated rats

  • Farhath Khanum
  • K.R. Anilakumar
  • K.R. Sudarshana Krishna
  • K.R. Viswanathan
  • K. Santhanam


Curry leaves are one of the spices used in Indian dishes for aroma and preservation. There are no reports on the antioxidant properties of curry leaves. In this study, the antioxidant potential of curry leaves in rats treated with a known chemical carcinogen, dimethylhydrazine hydrochloride (DMH) was investigated. Food intake was reduced in the rats fed curry leaf-supplemented diet but the body and the organ weights were not affected.Vitamin A content in the liver was significantly increased whereas glutathione (GSH) content was not altered. A 50% reduction was seen in the micronuclei induced by DMH and a 30% reduction in the activity of γ,-glutamyl transpeptidase when the rats were fed a curry leaf-supplemented diet. These results indicate that curry leaves have highpotential as reducer of the toxicity of DMH.

Antioxidant Carcinogenesis Catalase Curry leaves Dimethylhydrazine gamma-glutamyl transpeptidase Glutathione peroxidase Glutathione reductase Glutathione-S-trans-ferase GSH Micronuclei 


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  1. 1.
    Iyer UM, Mani UV (1990) Studies on the effect of curry leaves supplementation on lipid profile, glycated proteins and amino acids in non insulin dependent diabetic patients. Plant Food Hum Nutr 40: 275–282.Google Scholar
  2. 2.
    TheWealth of India. Publisher Council of Scientific and Industrial Research, New Delhi1962, pp 446–447.Google Scholar
  3. 3.
    Nadkarni AK (ed) (1976) Indian Materia Medica, Vol 1. Popular Prakashan Pvt. Ltd.Google Scholar
  4. 4.
    Khan BA, Abraham A, Leelamma S (1996) Biochemical response in rats to in addition of curry leaf (Murraya Koenigii) and mustard seeds (Brassica juncea) to the diet. Plant Food Hum Nutr 49: 295–299.Google Scholar
  5. 5.
    Girotti AW, Deziel M (1983) Photodynamic action of protoporphyrin on resealed erythrocyte membranes: Mechanism of trapped markers. In: Kessel D, Doughenty TJ (eds), Prophyrin Photo Sensitization. New York, Plenum Press, pp 213–225.Google Scholar
  6. 6.
    Ellman GL (1958) Liver glutathione. A colorimetric method for determining low concentrations of mercaptans. Arch Biochem Biophys 74: 443–450.Google Scholar
  7. 7.
    Dann WJ, Evelyn (1938) The determination of Vitamin A with the photoelectric colorimeter. Biochem J 32: 1008–1017.Google Scholar
  8. 8.
    Cohen G, Dembiec C, Marens J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34: 30–38.Google Scholar
  9. 9.
    Weiss C, Maker HS, Lehrer GM (1980) Sensitive fluorometric assays for glutathione peroxidase and reductase. Anal Biochem 106: 512–516.Google Scholar
  10. 10.
    Habig WB, Pabst MJ, Jokoby WB (1974) Glutathione S-transferases. J Biochem 249:7130–7139.Google Scholar
  11. 11.
    Meister A, Tate SS, Griffith OW (1981)-Glutamyl transpeptidase. Meth Enzymol 77:237–253.Google Scholar
  12. 12.
    Countryman PI, Heddle JA (1976) The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat Res 41: 321–332.Google Scholar
  13. 13.
    Asp NG, Johnson CG, Halimer H, Siljestrom M (1983) Rapid enzymatic assay of insoluble and soluble dietary fiber. J Agric Food Chem 31: 476–482.Google Scholar
  14. 14.
    Arya SS, Nateshan V, Parihar DB, Vijayraghavan PK (1979) Stability of carotenoids in dehydrated carrots. J Food Technol 14: 579–586.Google Scholar
  15. 15.
    WHO (1990) International Programme on Chemical Safety Environmental Health Criteria 109, WHO.Google Scholar
  16. 16.
    Kummet T, Meysten FL Jr (1983) Vitamin A: A potential inhibitor of human cancer. Semin Oncol 10: 281–289.Google Scholar
  17. 17.
    Schwartz J, Suda D, Light G (1986). Beta carotene is associated with the regression of hamster buccal pouch carcinoma and the induction of tumor necrosis factor in macrophages. Biochem Biophys Res Commun 136: 1130–1135.Google Scholar
  18. 18.
    Linder MC (1985) Nutrition and cancer prevention. In: Linder Mc (ed), Nutritional Biochemistry andMetabolism with Chemical Applications. New York: Elsevier, pp 347–368Google Scholar
  19. 19.
    Fiala S, Mohindra A, Kettering WG, Fiala AE, Morris HP (1976) Glutathione and gamma glutamyl transpeptidase in rat liver during chemical carcinogenesis. J Natl Cancer Inst 57: 591–598.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Farhath Khanum
    • 1
  • K.R. Anilakumar
    • 1
  • K.R. Sudarshana Krishna
    • 1
  • K.R. Viswanathan
    • 1
  • K. Santhanam
    • 1
  1. 1.Department of Biochemistry & Nutrition, Defence Food Research Laboratory, Siddhartha layoutMysoreIndia

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