Virus Genes

, Volume 19, Issue 3, pp 229–233 | Cite as

Characterization of the Structural-Protein-Coding Region of SAT 2 Type Foot-and-Mouth Disease Virus

  • Hester Gertruida Van Rensburg
  • Louis Hendrik Nel


The South African Territories (SAT) types of foot-and-mouth disease (FMD) virus show marked genomic and antigenic variation in sub-Saharan Africa that is to a large extent geographically determined. This has implications for selection of appropriate vaccine strains as well as the accuracy of laboratory diagnosis. However, adaptation of field isolates as vaccine strains is cumbersome, time consuming and expensive. We propose the construction of recombinant viruses in which specific antigenic determinants can be manipulated. To achieve this goal, the structural-protein-coding region of a SAT 2 vaccine strain, ZIM 7/83/2, was determined and compared with two other known SAT 2 P1 regions. Five hypervariable regions were identified of which four are situated within VP1. The cleavage sites for proteolytic processing differs from serotype A, while the junction between P1/2A is variable within the SAT 2 serotype. These differences could influence the construction of recombinant vaccines.

picornavirus foot-and-mouth disease virus SAT 2 (South African Territories) structural-protein-coding region 


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  1. 1.
    Berinstein A., Roivainen M., Hovi T., Mason P.W., and Baxt B., J Virol 69, 2664±2666, 1995.Google Scholar
  2. 2.
    Belsham G.J., Prog Biophys Molec Biol 60, 241±260, 1993.Google Scholar
  3. 3.
    Baxt B., Vakharia V., Moore D.M., Franke A.J., and Morgan D.O., J Virol 63, 2143±2151, 1989.Google Scholar
  4. 4.
    Crowther J.R., Rowe C.A., and Butcher R., Epidemiol Infect 111, 391±406, 1993.Google Scholar
  5. 5.
    Harley E.H., ``DAPSA ± a program for DNA and protein sequence analysis, version 4.31'', Department of Chemical Pathology, University of Cape Town, South Africa, 1998.Google Scholar
  6. 6.
    Hunter P., OIE Sci Tech Rev 15, 913±922, 1996.Google Scholar
  7. 7.
    Kitson J.D.A., McCahon D., and Belsham G., Virology 179, 26±34, 1990.Google Scholar
  8. 8.
    Mateu M.G., Martinez M.A., Capucci L., Andreu D., Giralt E., Sobrino F., Brocchi E., and Domingo E., J Gen Virol 71, 629± 637, 1990.Google Scholar
  9. 9.
    Mateu M.G., Virus Res 38, 1±24, 1995.Google Scholar
  10. 10.
    Palmenberg A., in Semler B.L. and Ehrenfeld E. (eds.) Molecular Aspects of Picornavirus Infection and Detection. ASM, Washington, DC, p. 211, 1989.Google Scholar
  11. 11.
    Rico-Hesse R., Pallansch M.A., Nottay B.K., Virology 160, 311±322, 1987.Google Scholar
  12. 12.
    Robertson B.H., Grubman M.J., Weddell G.N., Moore D.M., Welsh J.D., Fischer T., Dowbenko D.J., Yansura D.G., Small B., and Klein D.G.J., Virol 54, 651±660, 1985.Google Scholar
  13. 13.
    Sa-Carvalho D., Rieder E., Baxt B., Rodarte R., Tanuri T., and Mason P.W., J Virol 71, 5115±5123, 1997.Google Scholar
  14. 14.
    Thomson G.R. in Coetzer J.A.W., Thomson G.R., and Tustin R.C. (eds) Infectious Diseases of Livestock with Special Reference to Southern Africa. Oxford University Press, Cape Town, p. 825, 1994.Google Scholar
  15. 15.
    VoslooW., Kirkbride E., Bengis R.G., Keet A.F., and Thomson G.R., Epidemiol Infect 114, 203±218, 1995.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Hester Gertruida Van Rensburg
    • 1
  • Louis Hendrik Nel
    • 1
    • 2
  1. 1.Exotic Diseases DivisionOnderstepoort Veterinary InstituteSouth Africa
  2. 2.Department of Microbiology and Plant PathologyUniversity of PretoriaPretoriaSouth Africa

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