Journal of Applied Phycology

, Volume 12, Issue 2, pp 169–176 | Cite as

Use of the green alga Ulva rigida C. Agardh as an indicator species to reassess metal pollution in the Thermaikos Gulf, Greece, after 13 years

  • P. Malea
  • S. Haritonidis


The concentrations of metals (Mn, Pb, Fe, Zn, Cu, Cd,Co, Ni, Cr, Na, K, Ca, Mg) were determined in thegreen alga Ulva rigida, in the sediment andseawater of Thermaikos Gulf (Greece) during monthlysamplings in 1994–1995. This Gulf is the recipientof domestic and industrial effluents. Pb, Fe, Cu, Coand Cr concentrations in U. rigida at the studyarea were higher than those 13 years earlier andapparently came from different sources than those forZn, Cd and Ni. The relative abundance of metals inthe alga decreased in the order: Mg > Na > K >Ca > Pb > Fe > Mn > Zn > Cr, Cu > Ni >Co > Cd. Only Cu concentrations in the alga fromKalochori and Cd ones from Viamyl showed significantseasonal changes. Cu and Cd concentrations ingeneral followed the same pattern of variation, withminimum values in winter-spring. This pattern isdiscussed in relation to growth dynamics and tissueage. Only Pb concentrations in the alga showed asignificant positive correlation with concentrationsin the seawater. There were both positive andnegative correlations among some metals in the alga. It is concluded that U. rigida can be used as anindicator species, especially for Pb.

accumulation green alga metal pollution Thermaikos Gulf (Greece) Ulva rigida 


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  1. Brix H, Lyngby JE (1983) The distribution of some metallic elements in eelgrass (Zostera marina L.) and sediment in the Limfjord, Denmark. Estuar. coast. Shelf Sci. 16: 455-467.Google Scholar
  2. Brix H, Lyngby JE, Schierup HH (1983) Eelgrass (Zostera marina L.) as an indicator organism of trace metals in Limfjord, Denmark. Mar. envir. Res. 8: 165-181.Google Scholar
  3. Catsiki VA, Papathanassiou E (1993) The use of the chlorophyte Ulva lactuca (L.) as indicator organism of metal pollution. In: Proceedings of Cost-48 Symposium of Sub Group III. Macroalgae, Eutrophication and Trace Metal Cycling in Estuaries and Lagoons. Thessaloniki, Greece, 93-105.Google Scholar
  4. Collins MB (1981) Sediment yield studies of headwater catchments in Sussex, S-E. England. Earth Surface Processes 13: 25-36.Google Scholar
  5. Diamantopoulos E, Deligiannis A, Ziogouk, Kozlakidou S, Stylianou I, Bagiatis (1989). Quantitative feature of the Sewage of Thessaloniki. 6th Seminar for protection of the environment. Aquatic Recipients, Thessaloniki, Greece: 129-137.Google Scholar
  6. Diannelidis B-E, Delivopoulos SG (1999) Ultrastructural effects of heavy metals on the epidermal cells of Dictyota spiralis (Mont.) Hamel (Dictyotales, Phaeophyta). Int. J. Algae 1(3): 21-30.Google Scholar
  7. Djingova R, Kuleff I, Arpadjan S, Voulgaropoulos A, Sawidis T, Alexandrov S (1987) Neutron activation and atomic absorption analysis of Ulva lactuca and Gracilaria verrucosa from Thermaikos Gulf, Greece. Toxicol. Envir. Chem. 15: 149-158.Google Scholar
  8. Ganoulis J, Krestenitis J (1982) Choix du site optimum d'evacuàtion des eaux usées dans une région côtière. Societé Hydrotechnique de France, 17 Journeés de J 'Hydraulique.Google Scholar
  9. Güven KC, Topcuo-Glu S, Kut D, Esen N, Erenturk N, Saygi N, Cevher E, Guvener B, Ozturk B (1992) Metal uptake by Black sea algae. Bot. mar. 35: 337-340.Google Scholar
  10. Haritonidis S (1978) Contribution to the research of marine macrophyceae (Chloro-, Phaeo-and Rhodophyceae) of Thermaikos Gulf. PhD Thesis, Univ. Thessaloniki, Greece, 175 pp. (inGreek).Google Scholar
  11. Haritonidis S (1993) Introduction into eutrophication problems and metal distribution in Greek lagoons. In: Proceedings of COST-48 Symposium of SubGroup III. Macroalgae, Eutrophication and Trace Metal Cycling in Estuaries and Lagoons. Thessaloniki, Greece, 24-26 September, 154-163.Google Scholar
  12. Haritonidis S, Malea P (1995) Seasonal and local variation of Cr, Ni and Co concentrations in Ulva rigida C. Agardh and Enteromorpha linza (Linnaeus) from Thermaikos Gulf, Greece. Envir. Pollut. 89: 319-327.Google Scholar
  13. Haritonidis S, Malea P (1999a) Bioaccumulation of metals by the green alga Ulva rigida from Thermaikos Gulf, Greece. Envir. Pollut. 104: 365-372.Google Scholar
  14. Haritonidis S, Malea P (1999b) Seasonal and local variation of metals in seawater treated by different methods and in sediment of Thermaikos Gulf, Greece. Obelia 25: 89-109.Google Scholar
  15. Ho YB (1987) Ulva lactuca (Chlorophyta, Ulvales) in Hong Kong intertidal waters-its nitrogen and phosphorus contents and its use as a bioindicator of eutrophication. Asian. Mar. Biol. 4: 97-102.Google Scholar
  16. Ho YB (1990) Ulva lactuca as bioindicator of metal contamination in interdal waters in Hong Kong. Hydrobiologia 203: 73-81.Google Scholar
  17. Hortung H, Kress N, Friedlander M (1992) Trace element concentrations intertidal algae collected along the Mediterranean shore. Israel Fresen. Envir. Bull. 1: 84-89.Google Scholar
  18. Karez CS, Magalhaes VF, Pfeiffer WC, Amado Fillo GM (1994) Trace metal accumulation by algae in Sepetiba Bay, Brazil. Envir. Pollut. 83: 351-356.Google Scholar
  19. Malea P, Haritonidis S (1995) Local distribution and seasonal variation of Fe, Pb, Zn, Cu, Cd, Na, K, Ca and Mg concentrations in the seagrass Cymodocea nodosa (Ucria) Aschers. in the Antikyra Gulf, Greece. P.S.Z.N.I. mar. Ecol. 16: 41-56.Google Scholar
  20. Malea P, Haritonidis S (1999a) Metal content in Enteromorpha linza (Linnaeus) in Thermaikos Gulf (Greece). Hydrobiologia 394: 103-112.Google Scholar
  21. Malea P, Haritonidis S (1999b) Seasonal accumulation of metals by red alga Gracilaria verrucosa (Huds.) Papenf. from Thermaikos Gulf, Greece. J. appl. Phycol. 11: 503-509.Google Scholar
  22. Malea P, Haritonidis S, Stratis I (1994) Bioaccumulation of metals by Rhodophyta species at Antikyra Gulf (Greece) near an aluminium factory. Bot. mar. 37: 505-513.Google Scholar
  23. Malea P, Haritonidis S, Kevrekidis T (1995) Metal content of some green and brown seaweeds from Antikyra Gulf (Greece). Hydrobiologia 310: 19-31.Google Scholar
  24. Marcantonatos G (1990) Treatment and Disposal of AquaticWastes, Sewage, Industrial Effluents, Animal Residue. Athens, 738 pp.Google Scholar
  25. Munda IM, Hundnik V (1991) Trace metal content in some seaweeds from the Northern Adriatic. Bot. mar. 34: 241-249.Google Scholar
  26. Orfanidis SE, Lazaridou E, Seferlis E, Haritonidis S (1994) Eutrophication and the abundance of seaweeds in the biotope of Agia Triada, Gulf of Thessaloniki. In: Proceedings 5th National l. Symp. Oceanogr. Fish. 1: 67-70.Google Scholar
  27. Pulich WM (1980) Heavy metal accumulation by selected Halodule wrightii Asch. populations in the Corpus Christi Bay area. Mar. Sci. Univ. Tex. 23: 89-100.Google Scholar
  28. Sawidis Th, Voulgaropoulos AN (1986) Seasonal bioaccumulation of iron, cobalt and copper in marine algae from Thermaikos Gulf of the Northern Aegean Sea, Greece. Mar. envir. Res. 19: 39-47.Google Scholar
  29. Shiber JG (1980) Trace metals with seasonal considerations in coastal algae and molluscs from Beirut, Lebanon. Hydrobiologia 69: 147-162.Google Scholar
  30. Shiber J, Washburn E (1978) Lead, mercury and certain nutrient elements in Ulva lactuca (Linnaeus) from Ras Beirut, Lebanon. Hydrobiologia 61: 187-192.Google Scholar
  31. Talbot V, Chegwidden A (1982) Cadmium and other heavy metal concentrations in selected biota from Cockburn Sound, western Australia, Aust. J. mar. Freshwat. Res. 33: 779-788.Google Scholar
  32. Wahbeh M (1984) Levels of Zn, Mn, Mg, Fe and Cd in three species of seagrasses from Aqaba (Jordan). Aquat. Bot. 20: 179-183.Google Scholar
  33. Wahbeh MI, Mahasneh DM, Mahasneh I (1985) Concentrations of zinc, manganese, copper, cadmium, magnesium and iron in ten species of algae and seawater from Aqaba, Jordan. Mar. envir. Res. 16: 98-102.Google Scholar
  34. Wong MH, Kwok TT, Ho KC (1982) Heavy metal in Ulva lactuca collected within Tolo Harbour and almost landlocked sea. Hydrobiologia 16: 223-230.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • P. Malea
    • 1
  • S. Haritonidis
    • 1
  1. 1.School of Biology, Department of BotanyUniversity of ThessalonikiThessalonikiGreece

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