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Virus Genes

, Volume 19, Issue 2, pp 131–142 | Cite as

Molecular Characterization of an Isolate of Citrus Tristeza Virus that Causes Severe Symptoms in Sweet Orange

  • Zhong-Nan Yang
  • Deborah M. Mathews
  • J. Allan Dodds
  • T. Erik Mirkov
Article

Abstract

The complete sequence (19,249 nucleotides) of the genome of citrus tristeza virus (CTV) isolate SY568 was determined. The genome organization is identical to that of the previously determined CTV-T36 and CTV-VT isolates. Sequence comparisons revealed that CTV-SY568, a severe stem-pitting isolate from California, has more than 87% overall sequence identity with CTV-VT, a seedling yellows isolate from Israel. Although SY568 has an overall sequence identity of 81% with CTV-T36, a quick decline isolate from Florida, the sequence identity in the 3′ half of the genome is over 90% while the sequence identity in the 5′ half of the genome is as low as 56%. Based on the sequence alignments of these three isolates, sequences in the 3′ half of the genome are generally well conserved, while the sequences in the 5′ half are relatively divergent. Sequence data of independent overlapping clones from the CTV-SY568 genome revealed two regions with highly divergent sequences. In open reading frame 1b (RNA dependent RNA polymerase), there were 118 nucleotide differences that lead to 16 amino acid changes. In the open reading frame of the divergent coat protein gene, 5 amino acid changes result from 48 nucleotide differences. Most differences occurred in the third position of the codons, and resulted in silent amino acid substitutions. RNase protection assays demonstrated that most of the clones obtained are representative of the major RNA species of this isolate. Northern analysis indicated that CTV-SY568 accumulated more viral RNA including genomic and certain subgenomic RNAs than isolates VT or T36 in sweet orange.

citrus tristeza stem pitting SY568 closterovirus RNase protection 

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References

  1. 1.
    Bar-Joseph M., Marcus R., and Lee R.F., Ann Rev Phytopath 27, 291–316, 1989.Google Scholar
  2. 2.
    Garnsey S.M., Gumpf D.J., Roistacher C.N., Civerolo E.L., Lee R.F., Yokomi R.K., and Bar-Joseph M., Phytophylactica 19, 151–157, 1987.Google Scholar
  3. 3.
    Calavan E.C., Harjung M.K., Blue R.L., Roistacher C.N., Gumpf D.J., and Moore P.W., Proc 8th Conf IOCV. Riverside, USA. 69–75, 1980.Google Scholar
  4. 4.
    Costa A.S. and Muller G.W., Plant Dis 64, 538–541, 1980.Google Scholar
  5. 5.
    Roistacher C.N., Dodds J.A., and Bash J.A., Proc 10th Conf IOCV. Valencia, Spain. 91–100, 1988.Google Scholar
  6. 6.
    Roistacher C.N. and Moreno P., Proc 11th Conf IOCV. Riverside, USA. 7–19, 1991.Google Scholar
  7. 7.
    Jarupat T., Dodds J.A., and Roistacher C.N., Proc 10th Conf IOCV. Riverside USA, 39–45, 1988.Google Scholar
  8. 8.
    Moreno P., Guerri J., Ballesterolmos J.F., Albiach R., and Martinez M.E., Plant Path 42, 35–41, 1993.Google Scholar
  9. 9.
    Broadbent P., Brlansky R.H., and Indsto J., Plant Dis 80, 329–333, 1996.Google Scholar
  10. 10.
    Bar-Joseph M., Gumpf D.F., Dodds J.A., Rosner A., and Ginzburg I., Phytopathology 75, 195–198, 1985.Google Scholar
  11. 11.
    Karasev A.V., Boyko V.P., Gowda S., Nikolaeva O.V., Hilf M.E., Koonin E.V., Niblett C.L., Cline K., Gumpf D.J., Lee R.F., Garnsey S.M., Lewandowski D.J., and Dawson W.O., Virology 208, 511–520, 1995.Google Scholar
  12. 12.
    Mawassi M., Mietkiewska E., Gofman R., Yang G., and Bar-Joseph M., J Gen Virol 77, 2359–2364, 1996.Google Scholar
  13. 13.
    Karasev A.V., Nikolaeva O.V., Koonin E.V., Gumpf D.J., and Garnsey S.M., J Gen Virol 75, 1415–1422, 1994.Google Scholar
  14. 14.
    Pappu H.R., Karasev A.V., Anderson E.J., Pappu S.S., Hilf M.E., Febres V., Eckloff R.M.G., McCaffery M., Boyko V., Gowda S., Dolja V.V., and Koonin E.V., Virology 199, 35–46, 1994.Google Scholar
  15. 15.
    Febres V.J., Ashoulin L., Mawassi M., Frank A., Bar-Joseph M., Manjunath K.L., Lee R.F., and Niblett C.L., Phytopathology 86, 1331–1335, 1996.Google Scholar
  16. 16.
    Roistacher C.N., Bar-Joseph M., and Gumpf D.J., Plant Dis 68, 494–496, 1984.Google Scholar
  17. 17.
    Morris T.J. and Dodds J.A., Phytopathology 69, 854–858, 1979.Google Scholar
  18. 18.
    Yang Z.N. and Mirkov T.E., Phytopathology 87, 932–939, 1997.Google Scholar
  19. 19.
    Sambrook J., Fritsch E.F., and Maniatis T., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY. Cold Spring Harbor Laboratory Press, p6.1–6.21, 1989.Google Scholar
  20. 20.
    Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res 12, 387–395, 1984.Google Scholar
  21. 21.
    Kurath G., Rey M.E.C., and Dodds J.A., Virology 189, 233–244, 1992.Google Scholar
  22. 22.
    Winter E., Yamamoto F., Almoguera C., and Perucho M., Proc Natl Acad Sci USA 82, 7575–7579, 1985.Google Scholar
  23. 23.
    Jones J.D.G., Dunsmuir P., and Bedbrook J., EMBO J 4, 2411–2418, 1985.Google Scholar
  24. 24.
    Tsang S.S., Yin X., Guzzo-Arkuran C., Jones V.S., and Davison A.J., BioTechniques 14, 380–381, 1993.Google Scholar
  25. 25.
    Beckers T., Schmidt P., and Hilgard P., BioTechniques 16, 1074–1078, 1994.Google Scholar
  26. 26.
    Koetsier P.A., Schorr J., and Doerfler W., BioTechniques 15, 260–262, 1993.Google Scholar
  27. 27.
    Ingelbrecht I.L., Mandelbaum C.I., and Mirkov T.E., BioTechniques 25, 420–423, 1998.Google Scholar
  28. 28.
    Sekiya M.E., Lawrence S.D., McCaffery M., and Cline K., J Gen Virol 72, 1013–1020, 1991.Google Scholar
  29. 29.
    Agranovsky A.A., Koonin E.V., Boyko V.P., Maiss E., Frotschl R., Lunina N.A., and Atabekov J.G., Virology 198, 311–324, 1994.Google Scholar
  30. 30.
    Klaassen V.A., Boeshore M.L., Koonin E.V., Tian T., and Falk B.W., Virology 208, 99–110, 1995.Google Scholar
  31. 31.
    Mawassi M., Mietkiewska E., Hilf M.E., Ashoulin L., Karasev A.V., Gafny R., Lee R.F., Garnsey S.M. Dawson W.O., and Bar-Joseph M., Virology 214, 264–268, 1995.Google Scholar
  32. 32.
    Hilf M.E., Karasev A.V., Pappu H.R., Gumpf D.J., Niblett C.L., and Garnsey S.M., Virology 208, 576–582, 1995.Google Scholar
  33. 33.
    Karasev A.V., Kikolaeva O.V., Mushegian A.R., Lee R.F., and Dawson W.O., Virology 221, 199–207, 1996.Google Scholar
  34. 34.
    Dodds J.A., Jordan R.L., Roistacher C.N., and Jarupat, T., Intervirology 27, 177–188, 1987.Google Scholar
  35. 35.
    Dodds J.A., Jarupat T., Roistacher C.N., and Lee J.G., Phytophylactica 19, 131–137, 1987.Google Scholar
  36. 36.
    Mawassi M., Karasev A.V., Mietjiewska E., Gafny R., Lee R.F., Dawson W.O., and Bar-Joseph M., Virology 208, 383–387, 1995.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Zhong-Nan Yang
    • 1
  • Deborah M. Mathews
    • 2
  • J. Allan Dodds
    • 2
  • T. Erik Mirkov
    • 1
  1. 1.Dept. of Plant Pathology and MicrobiologyThe Texas A&M University Agricultural Experiment StationWeslaco
  2. 2.Dept. of Plant PathologyUniversity of California RiversideRiverside

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