, Volume 33, Issue 1–3, pp 213–219 | Cite as

Metabolism of amyloid precursor protein in COS cells transfected with a β-secretase candidate

  • Hisashi Koike
  • Zen Kouchi
  • Tadatoshi Kinouchi
  • Tatsuya Maeda
  • Hiroyuki Sorimachi
  • Takaomi C. Saido
  • Kei Maruyama
  • Akira Okuyama
  • Koichi Suzuki
  • Shoichi IshiuraEmail author


Thimet oligopeptidase (TOP) is a thiol- andmetallo-dependent peptidase and has been shown to beone of the β-secretase candidates. TOPexpressed in COS cells cleaved amyloid precursorprotein (APP) at the β-secretase site, and wefound a proteolytic product of APP called secretedform of APP by β-secretase (sAPPβ) in theconditioned media. Here we demonstrate thatsAPPβ was increased in conditioned media whenTOP was coexpressed in COS cells with APP and treatedwith an ADAM inhibitor SI-27. In addition, althoughTOP expressed in COS cell was localized at nuclei orGolgi apparatus, it exclusively colocalized at Golgiapparatus when APP was coexpressed with TOP.

Alzheimer's disease amyloid precursor protein β-secretase hydroxamate-based metalloprotease inhibitor thimet oligopeptidase 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Hisashi Koike
    • 1
    • 2
  • Zen Kouchi
    • 3
  • Tadatoshi Kinouchi
    • 4
  • Tatsuya Maeda
    • 2
  • Hiroyuki Sorimachi
    • 5
  • Takaomi C. Saido
    • 6
  • Kei Maruyama
    • 7
  • Akira Okuyama
    • 8
  • Koichi Suzuki
    • 2
  • Shoichi Ishiura
    • 9
    Email author
  1. 1.Department of Life Sciences, Graduate School of Arts and SciencesThe University of TokyoTokyoJapan
  2. 2.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan
  3. 3.Department of PhysiologyTokyo Women's Medical University School of MedicineTokyoJapan
  4. 4.Department of BiochemistryJichi Medical SchoolTochigiJapan
  5. 5.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  6. 6.Laboratory for Proteolytic NeuroscienceRIKEN Brain Science InstituteSaitamaJapan
  7. 7.Department of Molecular BiologyTokyo Metropolitan Institute of PsychiatryTokyoJapan
  8. 8.Banyu Tsukuba Research InstituteIbarakiJapan
  9. 9.Department of Life Sciences, Graduate School of Arts and SciencesThe University of TokyoTokyoJapan

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