Advertisement

Journal of Applied Phycology

, Volume 12, Issue 3–5, pp 543–547 | Cite as

Bioactivity in free-living and symbiotic cyanobacteria of the genus Nostoc

  • Raffaella Piccardi
  • Andrea Frosini
  • Mario R. Tredici
  • Maria C. Margheri
Article

Abstract

Fifty cyanobacterial strains from different habitats(symbioses, soil, fresh and marine waters) belongingto the genus Nostoc were cultured and tested forbioactivity. Thirty-seven strains were isolated in ourlaboratory, the remaining were supplied by officialculture collections. All the organisms were grownunder controlled laboratory conditions. The biomasseswere lyophilised and extracted with ethanol:water toobtain a hydrophilic extract and then withdichloromethane:isopropanol to obtain a lipophilicextract. Both crude extracts were tested forantifungal (against Penicillium expansum andRhizoctonia solani) and antibacterial activity(against Agrobacterium vitis, Escherichiacoli and Staphylococcus epidermidis), and fortoxicity against Artemia salina nauplii.Twenty-four strains showed activity against at leastone of the target organisms. Bioactivity was equallydistributed between lipophilic and hydrophilicextracts, and was mostly directed against fungi (15strains) and Artemia nauplii (12 strains);antibacterial activity was less frequent (8 strains).The presence of bioactivity was independent of thestrain origin.

antifungal and antibacterial compounds bioactivity cyanobacteria cytotoxicity Nostoc 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bloor S, England RR (1989) Antibiotic production by the cyanobacterium Nostoc muscorum. J. appl. Phycol. 1: 367-372.Google Scholar
  2. Bobzin SC, Moore RE (1993) Biosynthetic origin of [7.7]paracyclophanes from cyanobacteria. Tetrahedron 49: 7651-7656.Google Scholar
  3. Borowitzka MA (1999) Pharmaceuticals and agrochemicals from microalgae. In Cohen Z (ed.), Chemicals from Microalgae. Taylor & Francis, London, pp. 311-351.Google Scholar
  4. de Mulé MCZ, Caire GZ de, de Cano MS, de Halperin DR (1991) Bioactive compounds from Nostoc muscorum (cyanobacteria). Cytobios 66: 169-172.Google Scholar
  5. De Philippis R, Margheri MC, Pelosi E, Ventura S (1993) Exopolysaccharide production by a unicellular cyanobacterium isolated from a hypersaline habitat. J. appl. Phycol. 5: 387-394.Google Scholar
  6. Dodds WK, Gudder DA, Mollenhauer D (1995) The ecology of Nostoc. J. Phycol. 31: 2-18.Google Scholar
  7. Esser MT, Mori T, Mondor I, Sattentau QJ, Dey B, Berger EA, Boyd MR, Lifson JD (1999) Cyanovirin-N binds to gp120 to interfere with CD4-dependent human immunodeficiency virus type 1 virion binding, fusion, and infectivity but does not affect the CD4 binding site on gp120 or soluble CD4-induced conformational changes in gp120. J. Virol. 73: 4360-4371.Google Scholar
  8. Flores E, Wolk CP (1986) Production by filamentous, nitrogen-fixing cyanobacteria, of a bacteriocin and of other antibiotics that kill related strains. Arch. Microbiol. 145: 215-219.Google Scholar
  9. Geitler L (1932) Cyanophyceae. In Kolkwitz R (ed.), Rabenhorst's Kryptogamen-flora von Deutschland, Österreich und der Schweiz, Vol 14, Akademische Verlagsgesellschaft m.b.H., Leipzig.Google Scholar
  10. Gromov BV, Vepritskiy AA, Titova NN, Mamkayeva KA, Alexandrova OV (1991) Production of the antibiotic cyanobacterin LU-1 by Nostoc linckia CALU 892 (cyanobacterium). J. appl. Phycol. 3: 55-59.Google Scholar
  11. Hemscheidt T, Puglisi MP, Larsen LK, Patterson GML, Moore RE, Rios JL, Clardy J (1994) Structure and biosynthesis of borophycin, a new boeseken complex of boric acid from a marine strain of the blue-green alga Nostoc linckia. J. org. Chem. 59: 3467-3471.Google Scholar
  12. Jaki B, Orjala J, Sticher O (1999) A novel extracellular diterpenoid with antibacterial activity from the cyanobacterium Nostoc commune. J. nat. Prod. 62: 502-503.Google Scholar
  13. Keating KI (1978) Blue-green algal inhibition of diatom growth: transition from mesotrophic to eutrophic community structure. Science 199: 971-973.Google Scholar
  14. Lahti K, Ahtiainen J, Rapala J, Sivonen K, Niemelä SI (1995) Assessment of rapid bioassays for detecting cyanobacterial toxicity. Lett. appl. Microbiol. 21: 109-114.Google Scholar
  15. Moore RE, Patterson GML, Carmichael WW (1988) New pharmaceuticals from cultured blue-green algae. In Fautin DG (ed.), Biomedical Importance ofMarine Organisms, Vol 13.Mem. Cal. Acad. Sci., 143-150.Google Scholar
  16. Namikoshi M, Rinehart KL (1996) Bioactive compounds produced by cyanobacteria. J. ind. Microbiol. 17:373-384.Google Scholar
  17. Patterson GML (1996) Biotechnological applications of cyanobacteria. J. sci. ind. Res. 55: 669-684.Google Scholar
  18. Patterson GML, Baldwin CL, Bolis CM, Caplan FR, Karuso H, Larsen LK, Levine IA, Moore RE, Nelson CS, Tschappat KD, Tuang GD, Furusawa E, Furusawa S, Norton TR, Raybourne RB (1991) Antineoplastic activity of cultured blue-green algae (Cyanophyta). J. Phycol. 27: 530-536.Google Scholar
  19. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. gen. Microbiol. 111: 1-61.Google Scholar
  20. Schlegel I, Doan NT, Chazal N de, Smith GD (1999) Antibiotic activity of new cyanobacterial isolates from Australia and Asia against green algae and cyanobacteria. J. appl. Phycol. 10: 471-479.Google Scholar
  21. Skulberg OM (2000) Microalgae as a source of bioactive molecules-experience from cyanophyte research. J. appl. Phycol. 12: 341-348.Google Scholar
  22. Sivonen K, Carmichael WW, Namikoshi M, Rinehart KL, Dahlem AM, Niemela SI (1990) Isolation and characterization of hepatotoxic microcystin homologs from the filamentous freshwater cyanobacterium Nostoc sp. strain 152. Appl. environ. Microbiol. 56: 2650-2657.Google Scholar
  23. Smith GD, Doan NT (1999) Cyanobacterial metabolites with bioactivity against photosynthesis in cyanobacteria, algae and higher plants. J. appl. Phycol. 11: 337-344.Google Scholar
  24. Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD (1993) A microwell cytotoxicity assay using Artemia salina (Brine shrimp). Planta Med. 59: 250-252.Google Scholar
  25. Todorova AK, Jüttner F, Linden A, Plüss T, von Philipsborn W (1995) Nostocyclamide: a new macrocyclic, thiazole-containing allelochemical fron Nostoc sp. 31 (cyanobacteria). J. org. Chem. 60: 7891-7895.Google Scholar
  26. Tredici MR, Margheri MC, Giovannetti L, De Philippis R, Vincenzini M (1988) Heterotrophic metabolism and diazotrophic growth of Nostoc sp. from Cycas circinalis. Plant and Soil 110: 199-206.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Raffaella Piccardi
    • 1
  • Andrea Frosini
    • 1
  • Mario R. Tredici
    • 1
  • Maria C. Margheri
    • 1
  1. 1.Dipartimento di Biotecnologie AgrarieUniversità di Firenze and Centro di Studio dei Microrganismi Autotrofi, CNRFirenzeItaly

Personalised recommendations