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Journal of Applied Phycology

, Volume 10, Issue 5, pp 481–501 | Cite as

Cryopreservation of eukaryotic algae – a review of methodologies

  • Rebecca Taylor
  • Robert L. Fletcher
Article

Abstract

Since pioneering work in the early 1960s, there has been growing interest and numerous experimental investigations into the cryopreservation of algal material. Mostly, these studies relate to the requirement for long term preservation and storage of algal material contained in culture collections or used in the seaweed mariculture industry. The present review deals with techniques used in the cryopreservation of biological samples and their application to both micro- and macroalgae. Methods for the prevention of cell damage and freezing injury during the cooling and low-temperature storage of algal material are discussed with reference to the effect on viability of such variables as cooling rates, final temperatures attained, the use of various types and concentrations of cryoprotectants, thawing rates, and storage times and temperatures. Some consideration is also given to the various methods used for increasing cell viability, including the induction of freezing tolerance. Cryopreservation protocols employed by numerous workers in this field are detailed, and concluding remarks are made on those techniques and conditions providing optimum viability of cryopreserved algae.

cryopreservation microalgae macroalgae cryoprotectant cooling rate freezing tolerance storage 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Rebecca Taylor
    • 1
  • Robert L. Fletcher
    • 2
  1. 1.Department of Biological SciencesUniversity of DundeeDundeeUK
  2. 2.Institute of Marine SciencesUniversity of PortsmouthEastney, Portsmouth, HampshireUK

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