Cytotechnology

, Volume 29, Issue 2, pp 115–120 | Cite as

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Article

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

ginsenoside-Rb1 ELISA monoclonal antibody qualitative analysis mass spectrometry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Besso H, Kasai R, Saruwatari Y, Fuwa T and Tanaka O (1982) Ginsenoside-Ra1 and ginsenoside-Ra2, new dammarane-saponins of ginseng roots. Chem Pharm Bull 30: 2380-2385.Google Scholar
  2. Erlanger BF and Beiser, SM (1964) Antibodies specific for ribonucleosides and ribonucleotides and their reaction with DNA. Proc Natl Acad Sci US 52: 68-74.CrossRefGoogle Scholar
  3. Goto Y, Shima Y, Morimoto S, Shoyama Y, Murakami H, Kusai A and Nijima K (1994) Determination of tetrahydrocannabinolic acid-carrier protein conjugate by matrix-assisted laser desorption/ionization mass spectrometry and antibody formation. Org Mass Spectr 29: 668-671.CrossRefGoogle Scholar
  4. Hansen L and Boll PM (1986) Polyacetylenes in Araliaceae: Their chemistry, biosynthesis and biological significance. Phytochemistry 25: 285-293.CrossRefGoogle Scholar
  5. Ishiyama M, Shoyama Y, Murakami H and Shinohara H (1996) Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology 18: 153-158.CrossRefGoogle Scholar
  6. Kitagawa I, Taniyama T, Shibuya H, Noda T and Yoshikawa M (1987) Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2): Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi 107: 495-505.PubMedGoogle Scholar
  7. Koizumi H, Sanada S, Ida Y and Shoji J (1982) Studies on the saponins of Ginseng. IV. On the structure and enzymatic hydrolysis of ginsenoside-Ra1. Chem Pharm Bull 30: 2393-2398.Google Scholar
  8. Sakata R, Shoyama Y and Murakami H (1994) Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin. Cytotechn 16: 101-108.CrossRefGoogle Scholar
  9. Samukawa K, Yamashita H, Matsuda H and Kubo M (1995) Simultaneous analysis of ginsenosides of various ginseng radix by HPLC. Yakugaku Zasshi 115: 241-249.PubMedGoogle Scholar
  10. Sankawa U, Sung CK, Han BH, Akiyama T and Kawashima K (1982) Radioimmunoassay for the determination of ginseng saponin, ginsenoside Rg1. Chem. Pharm. Bull. 30: 1907-1910.Google Scholar
  11. Shibata S (1982) Chemistry of components in ginseng. J Traditional Sino-Japanese Medicine, 3: 62-69.Google Scholar
  12. Shoyama Y, Matsushita H, Zhu XX and Kishira H (1995) Somatic embryogenesis in ginseng (Panax species) in Biotechnology in Agriculture and Forestry Vol 31; Springer-Verlag, Berlin, pp. 343-356.Google Scholar
  13. Shoyama Y, Fukada T and Murakami H (1995) Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechn 19: 44-61.Google Scholar
  14. Shoyama Y, Zhu XX, Nakai R and Shiraishi S (1997) Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets. Plant Cell Rep 16: 450-453.Google Scholar
  15. Soldati F and Sticher O (1980) HPLC separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng drug preparations. 2nd communication Planta Medica 39: 348-357.CrossRefGoogle Scholar
  16. Sticher O and Solidati F (1979) HPLC separation and quantitative determination of ginsenosides from Panax giseng, Panax quinquefolium and from ginseng drug preparations. Planta Medica 36: 30-42.PubMedGoogle Scholar
  17. Tanaka O (1973) Metabolism and disease, Vol. 10, Nakayama Shoten, Tokyo.Google Scholar
  18. Tanaka H, Morimoto S and Shoyama Y (1993) Cannabis 21, Biotransformation of cannabinol to its glycosides by in vitro plant tissue. J Nat Prod 56: 2058-2072.Google Scholar
  19. Tani T, Kubo M, Katsuki, T, Higashino M, Hayashi T and Arichi S (1981) Histochemistry II. Ginsenosides in Ginseng (Panax ginseng, root). J Nat Prod 44: 401-407.CrossRefGoogle Scholar
  20. Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N and Takada K (1993) Characterization of two novel polysaccharides having immunological activities from the root of Panax ginseng. Chem Pharm Bull 16: 1097-1090.Google Scholar
  21. Weiler EW and Zenk MH (1976) Radioimmunoassay for the determination of digoxin and related compounds in Digitalis lanata. Phytochem 15: 1537-1545.CrossRefGoogle Scholar
  22. Yamaguchi H, Kasai R, Matsuura H, Tanaka O and Fuwa T (1988) High-performance liquid chromatographic analysis of acidic saponins of ginseng and related plants. Chem Pharm Bull 36: 3468-3473.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Hiroyuki Tanaka
    • 1
  • Noriko Fukuda
    • 1
  • Yukihiro Shoyama
    • 1
  1. 1.Department of Pharmacognosy, Faculty of Pharmaceutical SciencesKyushu UniversityHigashi-ku, FukuokaJapan

Personalised recommendations