, Volume 29, Issue 2, pp 115–120 | Cite as

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

  • Hiroyuki Tanaka
  • Noriko Fukuda
  • Yukihiro Shoyama


The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

ginsenoside-Rb1 ELISA monoclonal antibody qualitative analysis mass spectrometry 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Besso H, Kasai R, Saruwatari Y, Fuwa T and Tanaka O (1982) Ginsenoside-Ra1 and ginsenoside-Ra2, new dammarane-saponins of ginseng roots. Chem Pharm Bull 30: 2380-2385.Google Scholar
  2. Erlanger BF and Beiser, SM (1964) Antibodies specific for ribonucleosides and ribonucleotides and their reaction with DNA. Proc Natl Acad Sci US 52: 68-74.CrossRefGoogle Scholar
  3. Goto Y, Shima Y, Morimoto S, Shoyama Y, Murakami H, Kusai A and Nijima K (1994) Determination of tetrahydrocannabinolic acid-carrier protein conjugate by matrix-assisted laser desorption/ionization mass spectrometry and antibody formation. Org Mass Spectr 29: 668-671.CrossRefGoogle Scholar
  4. Hansen L and Boll PM (1986) Polyacetylenes in Araliaceae: Their chemistry, biosynthesis and biological significance. Phytochemistry 25: 285-293.CrossRefGoogle Scholar
  5. Ishiyama M, Shoyama Y, Murakami H and Shinohara H (1996) Production of monoclonal antibodies and development of an ELISA for solamargine. Cytotechnology 18: 153-158.CrossRefGoogle Scholar
  6. Kitagawa I, Taniyama T, Shibuya H, Noda T and Yoshikawa M (1987) Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2): Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi 107: 495-505.PubMedGoogle Scholar
  7. Koizumi H, Sanada S, Ida Y and Shoji J (1982) Studies on the saponins of Ginseng. IV. On the structure and enzymatic hydrolysis of ginsenoside-Ra1. Chem Pharm Bull 30: 2393-2398.Google Scholar
  8. Sakata R, Shoyama Y and Murakami H (1994) Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin. Cytotechn 16: 101-108.CrossRefGoogle Scholar
  9. Samukawa K, Yamashita H, Matsuda H and Kubo M (1995) Simultaneous analysis of ginsenosides of various ginseng radix by HPLC. Yakugaku Zasshi 115: 241-249.PubMedGoogle Scholar
  10. Sankawa U, Sung CK, Han BH, Akiyama T and Kawashima K (1982) Radioimmunoassay for the determination of ginseng saponin, ginsenoside Rg1. Chem. Pharm. Bull. 30: 1907-1910.Google Scholar
  11. Shibata S (1982) Chemistry of components in ginseng. J Traditional Sino-Japanese Medicine, 3: 62-69.Google Scholar
  12. Shoyama Y, Matsushita H, Zhu XX and Kishira H (1995) Somatic embryogenesis in ginseng (Panax species) in Biotechnology in Agriculture and Forestry Vol 31; Springer-Verlag, Berlin, pp. 343-356.Google Scholar
  13. Shoyama Y, Fukada T and Murakami H (1995) Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechn 19: 44-61.Google Scholar
  14. Shoyama Y, Zhu XX, Nakai R and Shiraishi S (1997) Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets. Plant Cell Rep 16: 450-453.Google Scholar
  15. Soldati F and Sticher O (1980) HPLC separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng drug preparations. 2nd communication Planta Medica 39: 348-357.CrossRefGoogle Scholar
  16. Sticher O and Solidati F (1979) HPLC separation and quantitative determination of ginsenosides from Panax giseng, Panax quinquefolium and from ginseng drug preparations. Planta Medica 36: 30-42.PubMedGoogle Scholar
  17. Tanaka O (1973) Metabolism and disease, Vol. 10, Nakayama Shoten, Tokyo.Google Scholar
  18. Tanaka H, Morimoto S and Shoyama Y (1993) Cannabis 21, Biotransformation of cannabinol to its glycosides by in vitro plant tissue. J Nat Prod 56: 2058-2072.Google Scholar
  19. Tani T, Kubo M, Katsuki, T, Higashino M, Hayashi T and Arichi S (1981) Histochemistry II. Ginsenosides in Ginseng (Panax ginseng, root). J Nat Prod 44: 401-407.CrossRefGoogle Scholar
  20. Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N and Takada K (1993) Characterization of two novel polysaccharides having immunological activities from the root of Panax ginseng. Chem Pharm Bull 16: 1097-1090.Google Scholar
  21. Weiler EW and Zenk MH (1976) Radioimmunoassay for the determination of digoxin and related compounds in Digitalis lanata. Phytochem 15: 1537-1545.CrossRefGoogle Scholar
  22. Yamaguchi H, Kasai R, Matsuura H, Tanaka O and Fuwa T (1988) High-performance liquid chromatographic analysis of acidic saponins of ginseng and related plants. Chem Pharm Bull 36: 3468-3473.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Hiroyuki Tanaka
    • 1
  • Noriko Fukuda
    • 1
  • Yukihiro Shoyama
    • 1
  1. 1.Department of Pharmacognosy, Faculty of Pharmaceutical SciencesKyushu UniversityHigashi-ku, FukuokaJapan

Personalised recommendations