Journal of Applied Phycology

, Volume 10, Issue 6, pp 569–572

Flow cytometric analysis of nocodazole-induced cell-cycle arrest in the pennate diatom Phaeodactylum tricornutum Bohlin

  • Christine K.F. Ng
  • Connie M.C. Lam
  • Patrick K.K. Yeung
  • J.T.Y. Wong
Article
  • 373 Downloads

Abstract

The microtubule inhibitor, nocodazole (2.5 mg L-1), can arrest the cell cycle of the pennate diatom Phaeodactylum tricornutum Bohlin at G2 + M phase. Flow cytometric analysis of cells treated with nocodazole suggest that the proportion of cells at G2 + M phase can accumulate to over 95%. Even after a 48-h treatment with nocodazole (2.5 mg L-1), the cells can still exit mitosis, suggesting that the cell-cycle arrest is reversible.

flow cytometry diatom Phaeodactylum tricornutum cell cycle nocodazole 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brzezinski MA, Olson RJ, Chisholm SW(1990) Silicon availability and cell-cycle progression in marine diatoms. Mar. Ecol. Progr. Ser. 67: 83-96.Google Scholar
  2. Chang J, Carpenter EJ (1994) Active growth of the oceanic dino-flagellate Ceratium teresin the Caribbean and Sargasso seas estimated by cell cycle analysis. J. Phycol. 30: 375-381.CrossRefGoogle Scholar
  3. Cohn SA, Pickett-Heaps JD (1988) The effects of colchicine and dinitrophenol on the in vitro rates of anaphase A and B in the diatom Surirella.Eur. J. Cell Biol. 46: 523-530.PubMedGoogle Scholar
  4. D'Elia E, Guillard RRL, Nelson DM (1979) Growth and competition of the marine diatoms Phaeodactylum tricornutumand Thalassiosira pseudonana. 1. Nutrient effects. Mar. Biol. 50: 305-312.Google Scholar
  5. Jacobs CW, Adams AEM, Szaniszlo PJ, Pringle JR (1988) Functions of microtubules in the Saccharomyces cerevisiaecell cycle. J. Cell Biol. 107: 1409-1426.PubMedCrossRefGoogle Scholar
  6. Jordan MA, Thrower D, Wilson L (1992) Effect of vinblastine, podophyllotoxin and nocodazole on mitotic spindles - Implications for the role of microtubule dynamics in mitosis. J. Cell Sci. 102: 401-416.PubMedGoogle Scholar
  7. Olson RJ, Vaulot D, Chisholm SW(1986) Effects of environmental stresses on the cell cycle of two marine phytoplankton species. Plant Physiol. 80: 918-925.PubMedCrossRefGoogle Scholar
  8. Pickett-Heaps JD (1991) Cell division in diatoms. Int. Rev. Cytol. 128: 61-108.Google Scholar
  9. Pickett-Heaps JD, Spurck TP (1982) Studies on kinetochore function in mitosis. I. The effects of colchicine and cytochalasin on mitosis in the diatom Hantzschia amphioxys.Eur. J. Cell Biol. 28: 77-82.PubMedGoogle Scholar
  10. Vaulot D, Olson RJ, Chisholm SW (1986a) Light and dark control of the cells cycle in two marine phytoplankton species. Exp.Cell. Res. 167: 38-52.PubMedCrossRefGoogle Scholar
  11. Vaulot D, Olson RJ, Chisholm SW (1986b) Cells cycle response to nutrient starvation in two marine phytoplankton species, Thalassiosira weissflogiiand Hymenomonas carterae. Mar. Biol. 95: 625-630.CrossRefGoogle Scholar
  12. Wordemann L, McDonald KL, Cande WZ (1986) The distribution of cytoplasmic microtubules throughout the cell cycle of the centric diatom Stephanopyxis turris: their role in nuclear migration and positioning the mitotic spindle during cytokinesis. J. Cell Biol. 102: 1688-1988.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Christine K.F. Ng
    • 1
  • Connie M.C. Lam
    • 1
  • Patrick K.K. Yeung
    • 1
  • J.T.Y. Wong
    • 1
  1. 1.Biology DepartmentHong Kong University of Science and TechnologyKowloon, Hong KongChina

Personalised recommendations