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Cytotechnology

, Volume 28, Issue 1–3, pp 81–89 | Cite as

A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy

  • G. Schoofs
  • T. J. Monica
  • J. Ayala
  • J. Horwitz
  • T. Montgomery
  • G. Roth
  • F. J. Castillo
Article

Abstract

We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells – which support the propagation of E1 deficient adenovirus – were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.

adenovirus HEK 293 perfusion serum free media suspension perfusion 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • G. Schoofs
    • 1
  • T. J. Monica
    • 1
  • J. Ayala
    • 1
  • J. Horwitz
    • 1
  • T. Montgomery
    • 1
  • G. Roth
    • 1
  • F. J. Castillo
    • 1
  1. 1.Fermentation and Cell Culture DevelopmentBiotechnology R&D (CMC), Berlex BiosciencesRichmondU.S.A

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