Improvement of microalgal photosynthetic productivity by reducing the content of light harvesting pigment

  • Yuji Nakajima
  • Ryohei Ueda
Article

Abstract

Microalgal productivity was examined using both a wild type and a phycocyanin-deficient mutant of Synechocystis PCC 6714 (PD-1). The culture was conducted at various light intensities under low and high cell densities in a continuous culture system. At low light intensity, photosynthetic productivity was almost the same for both low and high cell densities. However, at higher light intensities photosynthetic productivity was higher in mutant PD-1 than in the wild type. At 2000 μmol photon m−2 s−1 the productivity was 50% higher in mutant PD-1. This result is consistent with our first report (Nakajima & Ueda, 1997), which showed that photosynthetic productivity can be improved by reducing the light harvesting pigment content in high cell density cultures at high light intensities. It is concluded that the technology for reducing LHP content is a useful method for improving photosynthetic productivity in algal mass production.

dense algal suspension light-harvesting pigment photosynthesis productivity cyanobacterium Synechocystis PCC 6714 

References

  1. Benemann JR (1989) The future of microalgal biotechnology. In Cresswell RC, Rees TAV, Shah N (eds), Algal and Cyanobacterial Biotechnology. Longman Scientific & Technical, Harlow, UK, 317–337.Google Scholar
  2. Chen F (1996) High cell density culture of microalgae in heterotrophic growth. Tibtech November 14: 421–426.Google Scholar
  3. Chow WS, Anderson JM (1987) Photosynthetic response of Pisum saivum to an increase in irradiance during growth II. Thylakoid membrane component Aus. J. Plant Physiol. 14: 9–19.CrossRefGoogle Scholar
  4. Chow WS, Hope AB (1987) The stoichiometries of supermolecular complex in thyrakoid membranes from spinach chloroplasts. Aust. J. Plant Physiol. 14: 21–28.CrossRefGoogle Scholar
  5. Fujita Y, Murakami A (1987) Regulation of electron transport composition in cyanobacterial photosynthetic system: Stoichiometry among PS I and PS II complexes and their light harvesting antennae and Cyt b6-f complex. Pl. Cell Physiol. 28: 1547–1553.Google Scholar
  6. Garewal HS, Wasserman AR (1974) Triton X-100-4 m urea as an extraction medium for membrane proteins. I. Purification of chloroplast cytochrome b 559. Biochemistry. 13: 4063–4071.PubMedCrossRefGoogle Scholar
  7. Goldman JC (1979) Outdoor algal mass cultures II. Photosynthetic yield limitation. Water Research 13: 119–136.CrossRefGoogle Scholar
  8. Hattori A, Fujita Y (1959) Crystalline phycobilin chromoprotenoids obtained from blue-green alga, Tolypothrix tenuis. J. Biochem. 46: 633–644.Google Scholar
  9. Hiyama T, Ke B (1972) Difference spectra and extinction coefficients of P700. Biochim. Biophys. Acta 267: 160–171.PubMedCrossRefGoogle Scholar
  10. Lee YK (1990) Genetic and technological improvements with respect to mass cultivation of microalgae. In Nga BH, Lee YK (eds), Microbiology Applications in Food Biotechnology, Barking, UK, 61–73.Google Scholar
  11. Mackinney G (1941) Absorption of light by chlorophyll solutions. J. biol. Chem. 140: 315–322.Google Scholar
  12. Melis A (1990) Response of the photosynthetic apparatus in Dunaliella salina (green algae) to light stress. Plant Physiol. 93: 1433–1440.PubMedCrossRefGoogle Scholar
  13. Nakajima Y, Tsuzuki M, Ueda R (1998) Reduced photoinhibition of a phycocyanin deficient mutant of Synechocystis PCC 6714. J. appl. Phycol. 10Google Scholar
  14. Nakajima Y, Ueda R (1997) Improvement of photosynthesis in dense microalgal suspension by reduction of light harvesting pigments. J. appl. Phycol. 9: 503–510.Google Scholar
  15. Neale PJ, Melis A (1986) Algal photosynthetic membrane complexes and the photosynthesis-irradiance curve: A comparison of light-adaptation responses in Chlamydomonas reinhardtii (Chlorophyta). J. Phycol. 22: 531–538.Google Scholar
  16. Richmond A (1992) Mass production of microalgae: a model of industrial photosynthesis. In Barber J, Guerrero MG, Medrano H (eds) Trends in Photosynthesis Research. Hampshire, UK, 305–317.Google Scholar
  17. Sukenik A, Falkowski PG, Benett J (1987) Potential enhancement of photosynthetic energy conversion in algal mass culture. Biotechnol. Bioengng 30: 970–977.CrossRefGoogle Scholar
  18. Sukenik A, Benett J, Falkowski P (1987) Light-saturated photosynthesis – limitation by electron transport or carbon fixation? Biochem. Biophys. Acta 897: 205–215.Google Scholar
  19. Watanabe A (1960) List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. gen. Aappl. Microbiol. 6: 283–292.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Yuji Nakajima
    • 1
  • Ryohei Ueda
    • 1
  1. 1.Bio Technology Research Laboratory, Advanced Technology Research CenterMitsubishi Heavy Industries, Ltd.YokohamaJapan

Personalised recommendations